000166145 001__ 166145
000166145 005__ 20240229123221.0
000166145 037__ $$aDKFZ-2020-02653
000166145 1001_ $$0P:(DE-He78)8e87b165655e54a5f7ce16d2ce01540e$$aCamazzola, Gianmarco$$b0$$udkfz
000166145 245__ $$aModulation of intracellular oxygen:making the cell nucleus hypoxic forextracellular oxic conditions
000166145 260__ $$c2020
000166145 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000166145 3367_ $$02$$2EndNote$$aThesis
000166145 3367_ $$2BibTeX$$aMASTERSTHESIS
000166145 3367_ $$2DRIVER$$amasterThesis
000166145 3367_ $$0PUB:(DE-HGF)19$$2PUB:(DE-HGF)$$aMaster Thesis$$bmaster$$mmaster$$s1608296646_32509
000166145 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000166145 502__ $$aMasterarbeit, Universität Heidelberg, 2020$$bMasterarbeit$$cUniversität Heidelberg$$d2020$$gFakultät für Physik und Astronomie
000166145 520__ $$aIn the presented work the analysis of a novel molecular mechanism is proposed, by whichcells can modulate their intracellular oxygen and potentially become hypoxic, inducingfavourable circumstances for metabolic reprogramming, even under extracellular oxic conditions. This phenomenon is based on the competition between O2 diffusion, from theextracellular environment to the nucleus, and its consumption, especially by NOX enzymes.The novelty revolves around the assumptions that the endoplasmic reticulum (ER) membranes can lower the diffusion rate of oxygen, and that the sensing enzymes become moreeffective at removing O2for low values of the Michaelis constant KM. After a preliminarystudy of oxygen concentration inside the cell, an analysis of different factors affecting thisphenomenon was carried out. Attention was given to the Michaelis-Menten parametersVmax and KM, considering consumption differences between ER and cytosol, the extracellular oxygen, the number of membrane pairs, the membrane porosity and the dependence onthe cell cycle phase. A relation between porosity and number of membranes was established,and the ratio of nuclear O2 of G1 phase relative to S phase, for different KM, was determined. In conclusion, the initial hypothesis has been confirmed, and some experimentalresults obtained in previous studies have been validated.
000166145 536__ $$0G:(DE-HGF)POF3-315$$a315 - Imaging and radiooncology (POF3-315)$$cPOF3-315$$fPOF III$$x0
000166145 909CO $$ooai:inrepo02.dkfz.de:166145$$pVDB
000166145 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)8e87b165655e54a5f7ce16d2ce01540e$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000166145 9131_ $$0G:(DE-HGF)POF3-315$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vImaging and radiooncology$$x0
000166145 9141_ $$y2020
000166145 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000166145 980__ $$amaster
000166145 980__ $$aVDB
000166145 980__ $$aI:(DE-He78)E041-20160331
000166145 980__ $$aUNRESTRICTED