000166147 001__ 166147
000166147 005__ 20240229123221.0
000166147 037__ $$aDKFZ-2020-02655
000166147 1001_ $$0P:(DE-He78)dca2a002b12e5b64e0b3bba4bfd51ec4$$aVoigt, Jonah$$b0$$udkfz
000166147 245__ $$aDetection of Radiation Induced H2O2 in H460Lung Cancer Cells Using Novel NucPE1 Dye
000166147 260__ $$c2020
000166147 3367_ $$2DRIVER$$abachelorThesis
000166147 3367_ $$02$$2EndNote$$aThesis
000166147 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000166147 3367_ $$0PUB:(DE-HGF)2$$2PUB:(DE-HGF)$$aBachelor Thesis$$bbachelor$$mbachelor$$s1608295435_1711
000166147 3367_ $$2BibTeX$$aMASTERSTHESIS
000166147 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000166147 502__ $$aBachelorarbeit, Universität Heidelberg, 2020$$bBachelorarbeit$$cUniversität Heidelberg$$gFakultät für Physik und Astronomie
000166147 520__ $$aThe tumor response after irradiation depends largely on the applied dose and thereby the energy transferred to the cells. A possible newway to measure this deposited energy was found with the development of thenuclear-localized fluorescent hydrogen peroxide (H2O2) probe Nuclear PeroxyEmerald 1 (NucPE1). It is capable of detecting H2O2 inside the nucleus, areactive oxygen species (ROS) that is often induced by radiation and playsan important role in the production of indirect DNA damage. By measuringthe fluorescent signal of the nuclei of cells incubated with NucPE1 and treatedwith ionizing radiation, it can be possible to gain valuable insight into thisdose-response relationship.In this thesis, some of the general characteristics of NucPE1 were examined,including its stability in solution and its interaction with artificially addedH2O2. The time consistency of the fluorescent NucPE1 signal from H460 lungcancer cells was measured, and different protocols were investigated that couldpotentially increase its lifetime. A protocol to freeze and fix the NucPE1 stainedcells was developed, which allowed measurement of a rather stable signal for upto 45min after irradiation. The fluorescent intensities of H460 cells irradiatedwith different doses were measured using confocal microscopy and the nuclei ofstained cells were extracted and analyzed using flow cytometry (FACS). ThisFACS analysis led to the most promising results that almost fit the expectationof a linear-quadratic NucPE1-dose-response. With this, a large step towardsthe usage of NucPE1 as a chemical dosimeter has been achieved.
000166147 536__ $$0G:(DE-HGF)POF3-315$$a315 - Imaging and radiooncology (POF3-315)$$cPOF3-315$$fPOF III$$x0
000166147 909CO $$ooai:inrepo02.dkfz.de:166147$$pVDB
000166147 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)dca2a002b12e5b64e0b3bba4bfd51ec4$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000166147 9131_ $$0G:(DE-HGF)POF3-315$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vImaging and radiooncology$$x0
000166147 9141_ $$y2020
000166147 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000166147 980__ $$abachelor
000166147 980__ $$aVDB
000166147 980__ $$aI:(DE-He78)E041-20160331
000166147 980__ $$aUNRESTRICTED