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@MASTERSTHESIS{Voigt:166147,
      author       = {J. Voigt$^*$},
      title        = {{D}etection of {R}adiation {I}nduced {H}2{O}2 in
                      {H}460{L}ung {C}ancer {C}ells {U}sing {N}ovel {N}uc{PE}1
                      {D}ye},
      school       = {Universität Heidelberg},
      type         = {Bachelorarbeit},
      reportid     = {DKFZ-2020-02655},
      year         = {2020},
      note         = {Bachelorarbeit, Universität Heidelberg, 2020},
      abstract     = {The tumor response after irradiation depends largely on the
                      applied dose and thereby the energy transferred to the
                      cells. A possible newway to measure this deposited energy
                      was found with the development of thenuclear-localized
                      fluorescent hydrogen peroxide (H2O2) probe Nuclear
                      PeroxyEmerald 1 (NucPE1). It is capable of detecting H2O2
                      inside the nucleus, areactive oxygen species (ROS) that is
                      often induced by radiation and playsan important role in the
                      production of indirect DNA damage. By measuringthe
                      fluorescent signal of the nuclei of cells incubated with
                      NucPE1 and treatedwith ionizing radiation, it can be
                      possible to gain valuable insight into thisdose-response
                      relationship.In this thesis, some of the general
                      characteristics of NucPE1 were examined,including its
                      stability in solution and its interaction with artificially
                      addedH2O2. The time consistency of the fluorescent NucPE1
                      signal from H460 lungcancer cells was measured, and
                      different protocols were investigated that couldpotentially
                      increase its lifetime. A protocol to freeze and fix the
                      NucPE1 stainedcells was developed, which allowed measurement
                      of a rather stable signal for upto 45min after irradiation.
                      The fluorescent intensities of H460 cells irradiatedwith
                      different doses were measured using confocal microscopy and
                      the nuclei ofstained cells were extracted and analyzed using
                      flow cytometry (FACS). ThisFACS analysis led to the most
                      promising results that almost fit the expectationof a
                      linear-quadratic NucPE1-dose-response. With this, a large
                      step towardsthe usage of NucPE1 as a chemical dosimeter has
                      been achieved.},
      cin          = {E041},
      cid          = {I:(DE-He78)E041-20160331},
      pnm          = {315 - Imaging and radiooncology (POF3-315)},
      pid          = {G:(DE-HGF)POF3-315},
      typ          = {PUB:(DE-HGF)2},
      url          = {https://inrepo02.dkfz.de/record/166147},
}