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@ARTICLE{Silva:166554,
      author       = {V. C. Silva and A. M. G. S. Silva and J. A. D. Basílio and
                      J. A. Xavier and T. G. do Nascimento and R. M. Z. G. Naal
                      and M. P. Del Lama and L. A. D. Leonelo and N. L. O. N.
                      Mergulhão and F. C. A. Maranhão and D. M. W. Silva and R.
                      Owen and I. F. B. Duarte and L. C. G. Bulhões and I. D.
                      Basílio and M. O. F. Goulart},
      title        = {{N}ew {I}nsights for {R}ed {P}ropolis of
                      {A}lagoas-{C}hemical {C}onstituents, {T}opical {M}embrane
                      {F}ormulations and {T}heir {P}hysicochemical and
                      {B}iological {P}roperties.},
      journal      = {Molecules},
      volume       = {25},
      number       = {24},
      issn         = {1420-3049},
      address      = {Basel},
      publisher    = {MDPI44576},
      reportid     = {DKFZ-2020-02997},
      pages        = {5811},
      year         = {2020},
      abstract     = {The main objectives of this study were to evaluate the
                      chemical constitution and allergenic potential of red
                      propolis extract (RPE). They were evaluated, using high
                      performance liquid chromatography (HPLC) and the release of
                      β-hexosaminidase, respectively. A plethora of biologically
                      active polyphenols and the absence of allergic responses
                      were evinced. RPE inhibited the release of
                      β-hexosaminidase, suggesting that the extract does not
                      stimulate allergic responses. Additionally, the
                      physicochemical properties and antibacterial activity of
                      hydrogel membranes loaded with RPE were analyzed.
                      Bio-polymeric hydrogel membranes (M) were obtained using
                      $5\%$ carboxymethylcellulose (M1 and M2), $1.0\%$ of citric
                      acid (M3) and $10\%$ RPE (for all). Their characterization
                      was performed using thermal analysis, Fourier transform
                      infrared (FTIR), total phenolic content, phenol release test
                      and, antioxidant activity through
                      2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and Ferric
                      Reducing Antioxidant Power (FRAP). The latter appointed to
                      the similar antioxidant capacity of the M1, M2 and M3. The
                      degradation profiles showed higher thermostability to M3,
                      followed by M2 and M1. The incorporation of RPE into the
                      matrices and the crosslinking of M3 were evinced by FTIR.
                      There were differences in the release of phenolic compounds,
                      with a higher release related to M1 and lower in the
                      strongly crosslinked M3. The degradation profiles showed
                      higher thermostability to M3, followed by M2 and M1. The
                      antibacterial activity of the membranes was determined using
                      the disc diffusion assay, in comparison with controls,
                      obtained in the same way, without RPE. The membranes
                      elicited antibacterial activity against Staphylococcus
                      aureus and Staphylococcus epidermidis, with superior
                      performance over M3. The hydrogel membranes loaded with RPE
                      promote a physical barrier against bacterial skin infections
                      and may be applied in the wound healing process.},
      keywords     = {allergenic activity (Other) / anti-staphylococcal (Other) /
                      biopolymer (Other) / chromatographic profile (Other) /
                      phytochemical screening (Other) / sodium
                      carboxymethylcellulose (Other)},
      cin          = {C120},
      ddc          = {540},
      cid          = {I:(DE-He78)C120-20160331},
      pnm          = {313 - Cancer risk factors and prevention (POF3-313)},
      pid          = {G:(DE-HGF)POF3-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33317120},
      doi          = {10.3390/molecules25245811},
      url          = {https://inrepo02.dkfz.de/record/166554},
}