TY  - JOUR
AU  - Garcia-Moure, Marc
AU  - Gonzalez-Huarriz, Marisol
AU  - Labiano, Sara
AU  - Guruceaga, Elizabeth
AU  - Bandres, Eva
AU  - Zalacain, Marta
AU  - Marrodan, Lucia
AU  - de Andrea, Carlos E
AU  - Villalba Esparza, María
AU  - Martínez-Vélez, Naiara
AU  - Laspidea, Virginia
AU  - Puigdelloses, Montserrat
AU  - Gallego Perez-Larraya, Jaime
AU  - Iñigo-Marco, Ignacio
AU  - Stripecke, Renata
AU  - Chan, Jennifer A
AU  - Raabe, Eric H
AU  - Kool, Marcel
AU  - Gomez-Manzano, Candelaria
AU  - Fueyo, Juan
AU  - Patiño-García, Ana
AU  - Alonso, Marta M
TI  - Delta-24-RGD, an oncolytic adenovirus, increases survival and promotes proinflammatory immune landscape remodeling in models of AT/RT and CNS-PNET.
JO  - Clinical cancer research
VL  - 27
IS  - 6
SN  - 1557-3265
CY  - Philadelphia, Pa. [u.a.]
PB  - AACR
M1  - DKFZ-2021-00004
SP  - 1807-1820
PY  - 2021
N1  - 2021 Mar 15;27(6):1807-1820
AB  - Atypical teratoid/rhabdoid tumors (AT/RTs) and primitive neuroectodermal tumors (CNS-PNETs) are pediatric brain tumors with poor survival and life-long negative side effects. Here, the aim was to characterize the efficacy and safety of the oncolytic adenovirus Delta-24-RGDs, which selectively replicates in and kills tumor cells.Delta-24-RGD determinants for infection and replication were evaluated in patient expression data sets. Viral replication and cytotoxicity were assessed invitro in a battery of CNS-PNET and AT/RT cell lines. In vivo, efficacy was determined in different orthotopic mouse models, including early and established tumor models, a disseminated AT/RT lesion model and immunocompetent humanized mouse models (hCD34+-NSG-SGM3).Delta-24-RGD infected and replicated efficiently in all the cell lines tested. In addition, the virus induced dose-dependent cytotoxicity (IC50 below 1 PFU/cell) and the release of immunogenic markers. Invivo, a single intratumoral Delta-24-RGD injection (107 or 108 PFU) significantly increased survival and led to long-term survival in AT/RT and PNET models. Delta-24-RGD hindered the dissemination of AT/RTs and increased survival, leading to 70
LB  - PUB:(DE-HGF)16
C6  - pmid:33376098
DO  - DOI:10.1158/1078-0432.CCR-20-3313
UR  - https://inrepo02.dkfz.de/record/166637
ER  -