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@ARTICLE{Bhardwaj:167344,
      author       = {M. Bhardwaj$^*$ and T. Terzer$^*$ and P. Schrotz-King$^*$
                      and H. Brenner$^*$},
      title        = {{C}omparison of {P}roteomic {T}echnologies for
                      {B}lood-{B}ased {D}etection of {C}olorectal {C}ancer.},
      journal      = {International journal of molecular sciences},
      volume       = {22},
      number       = {3},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {DKFZ-2021-00291},
      pages        = {1189},
      year         = {2021},
      note         = {#EA:C070#LA:C070#},
      abstract     = {Blood-based protein biomarkers are increasingly being
                      explored as supplementary or efficient alternatives for
                      population-based screening of colorectal cancer (CRC). The
                      objective of the current study was to compare the diagnostic
                      potential of proteins measured with different proteomic
                      technologies. The concentrations of protein biomarkers were
                      measured using proximity extension assays (PEAs), liquid
                      chromatography/multiple reaction monitoring-mass
                      spectrometry (LC/MRM-MS) and quantibody microarrays (QMAs)
                      in plasma samples of 56 CRC patients and 99 participants
                      free of neoplasms. In another approach, proteins were
                      measured in serum samples of 30 CRC cases and 30
                      participants free of neoplasm using immunome full-length
                      functional protein arrays (IpAs). From all the measurements,
                      9, 6, 35 and 14 protein biomarkers overlapped for
                      comparative evaluation of (a) PEA and LC/MRM-MS, (b) PEA and
                      QMA, (c) PEA and IpA, and (d) LC/MRM-MS and IpA
                      measurements, respectively. Correlation analysis was
                      performed, along with calculation of the area under the
                      curve (AUC) for assessing the diagnostic potential of each
                      biomarker. DeLong's test was performed to assess the
                      differences in AUC. Evaluation of the nine biomarkers
                      measured with PEA and LC/MRM-MS displayed correlation
                      coefficients >+0.6, similar AUCs and DeLong's p-values
                      indicating no differences in AUCs for biomarkers like
                      insulin-like growth factor binding protein 2 (IGFBP2),
                      matrix metalloproteinase 9 (MMP9) and serum paraoxonase
                      lactonase 3 (PON3). Comparing six proteins measured with PEA
                      and QMA showed good correlation and similar diagnostic
                      performance for only one protein, growth differentiation
                      factor 15 (GDF15). The comparison of 35 proteins measured
                      with IpA and PEA and 14 proteins analyzed with IpA and
                      LC/MRM-MS revealed poor concordance and comparatively better
                      AUCs when measured with PEA and LC/MRM-MS. The comparison of
                      different proteomic technologies suggests the superior
                      performance of novel technologies like PEA and LC/MRM-MS
                      over the assessed array-based technologies in
                      blood-protein-based early detection of CRC.},
      keywords     = {LC/MRM-MS (Other) / biomarkers (Other) / colorectal cancer
                      (Other) / diagnosis (Other) / microarray (Other) / plasma
                      proteins (Other) / proximity extension assays (Other)},
      cin          = {C120 / C070 / HD01 / C060},
      ddc          = {540},
      cid          = {I:(DE-He78)C120-20160331 / I:(DE-He78)C070-20160331 /
                      I:(DE-He78)HD01-20160331 / I:(DE-He78)C060-20160331},
      pnm          = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
      pid          = {G:(DE-HGF)POF4-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33530402},
      doi          = {10.3390/ijms22031189},
      url          = {https://inrepo02.dkfz.de/record/167344},
}