000167447 001__ 167447
000167447 005__ 20240229133540.0
000167447 020__ $$a978-1-0716-1055-8 (print)
000167447 020__ $$a978-1-0716-1056-5 (electronic)
000167447 0247_ $$2doi$$a10.1007/978-1-0716-1056-5_13
000167447 0247_ $$2pmid$$apmid:33576978
000167447 0247_ $$2ISSN$$a1064-3745
000167447 0247_ $$2ISSN$$a1940-6029
000167447 0247_ $$2doi$$adoi: 10.1007/978-1-0716-1056-5_13. 
000167447 0247_ $$2doi$$adoi: 10.1007/978-1-0716-1056-5_13.
000167447 037__ $$aDKFZ-2021-00351
000167447 041__ $$aeng
000167447 082__ $$a570
000167447 1001_ $$aKönig, Courtney$$b0
000167447 245__ $$aMetastatic Dissemination Mimicked in a Multicellular Transwell Assay.
000167447 260__ $$a[Heidelberg]$$b[Springer]$$c2021
000167447 3367_ $$2DRIVER$$aarticle
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000167447 3367_ $$0PUB:(DE-HGF)3$$2PUB:(DE-HGF)$$aBook$$mbook
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000167447 3367_ $$2BibTeX$$aARTICLE
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000167447 3367_ $$00$$2EndNote$$aJournal Article
000167447 500__ $$a#LA:A190#DKFZ-ZMBH Alliance2021;2235:181-190
000167447 520__ $$aTumor progression and metastasis are multistep processes that are critically dependent on the interaction of metastasizing tumor cells with other cells of the local microenvironment. Mimicking the single steps of the metastatic cascade in vitro is therefore challenging when investigating not only tumor cell behavior alone but also cellular crosstalk between different cell populations. In particular, the crosstalk of tumor cells with pericytes and endothelial cells when accessing the bloodstream is of great importance for successful intravasation and metastatic dissemination. To examine metastatic intravasation and analyze the interaction of tumor cells with pericytes, which reside within the basement membrane and endothelial cells, aligning the vessel wall, we have designed a 3D in vitro transwell assay mimicking tumor cell intravasation into a vessel. Modifying the Boyden chamber transwell assay by seeding first an endothelial cell layer onto the transwell membrane and covering it with pericytes before adding the tumor cells allows us to investigate the role of pericytes and endothelial cells on tumor cell intravasation and at the same time to study their crosstalk at the molecular level and how this interaction influences tumor cell behavior. It further allows the manipulation of the system for proof-of-principle experimentation.
000167447 536__ $$0G:(DE-HGF)POF4-311$$a311 - Zellbiologie und Tumorbiologie (POF4-311)$$cPOF4-311$$fPOF IV$$x0
000167447 588__ $$aDataset connected to CrossRef Book Series, PubMed,
000167447 650_7 $$2Other$$aBoyden chamber
000167447 650_7 $$2Other$$aEndothelial cells
000167447 650_7 $$2Other$$aIntravasation
000167447 650_7 $$2Other$$aMulticellular crosstalk
000167447 650_7 $$2Other$$aPericytes
000167447 650_7 $$2Other$$aTumor cells
000167447 7001_ $$0P:(DE-He78)cc84101e4a6f5a7a41c9012f423dbd0f$$aRunge, Anja$$b1$$eLast author$$udkfz
000167447 773__ $$0PERI:(DE-600)2493551-7$$a10.1007/978-1-0716-1056-5_13.$$p181-190$$tMethods in molecular biology$$v2235$$x1064-3745$$y2021
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000167447 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)cc84101e4a6f5a7a41c9012f423dbd0f$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000167447 9130_ $$0G:(DE-HGF)POF3-311$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vSignalling pathways, cell and tumor biology$$x0
000167447 9131_ $$0G:(DE-HGF)POF4-311$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vZellbiologie und Tumorbiologie$$x0
000167447 9141_ $$y2021
000167447 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2020-09-11
000167447 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2020-09-11
000167447 9201_ $$0I:(DE-He78)A190-20160331$$kA190$$lA190 Vaskuläre Onkologie und Metastatistierung$$x0
000167447 980__ $$ajournal
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000167447 980__ $$abook
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