TY  - JOUR
AU  - Pavez Loriè, Elizabeth
AU  - Boukamp, Petra
TI  - Methods in cell biology: Cell-derived matrices.
JO  - Methods in cell biology
VL  - 156
SN  - 0091-679X
CY  - New York, NY [u.a.]
PB  - Elsevier
M1  - DKFZ-2021-00368
SP  - 309-332
PY  - 2020
N1  - Volume 156, 2020, Pages 309-332#LA:A110#
AB  - Three-dimensional (3D) in vitro skin and skin cancer models have become an invaluable tool in skin research. They go back to 1979, when Bell and colleagues reported on the establishment of a fibroblast-dependent collagen tissue (Bell, Ivarsson, </td><td width="150">
AB  -  Merrill, 1979). On top of such tissue a stratified and differentiated epidermis could be established (Bell, Merrill, </td><td width="150">
AB  -  Solomon, 1979). Hydrogel-based dermal equivalents have been generated ever since and upon co-culture with normal human skin keratinocytes, these constructs were then termed skin equivalents. Due to a number of deficiencies, the most important one being their restricted survival time, new developments helped to circumvent premature fibroblast activation and tissue destruction. By avoiding collagen for the dermal equivalent (DE), we proposed, a scaffold-based DE, allowing fibroblasts to reorganize the primary fibrin solution into an 'authentic' dermal matrix (Boehnke et al., 2007; Stark et al., 2004, 2006). With this, our goal of a long-term skin equivalent-successful cultivation for several months-was achieved. Nevertheless, also this model presented limitations. One being its opaqueness made it difficult to image the intact tissue. Another draw-back was that tumor cells upon invasion used the scaffold as a guardrail leaving behind an unspecific invasion pattern. All this could be avoided by an approach, the fibroblast-derived matrix-based model, based on the work by Ahlfors and Billiar (2007) We here provide a protocol for this type of model, thereby providing the basis for future work in the field of skin research.
KW  - Cells, Cultured
KW  - Cytological Techniques: methods
KW  - Extracellular Matrix: metabolism
KW  - Fibroblasts: cytology
KW  - Fibroblasts: metabolism
KW  - Humans
KW  - Keratinocytes: cytology
KW  - Keratinocytes: metabolism
KW  - 3D models (Other)
KW  - Cell-derived matrix (Other)
KW  - Dermal equivalent (Other)
KW  - ECM (Other)
KW  - Fibroblast (Other)
KW  - Human model (Other)
KW  - Keratinocyte (Other)
KW  - Long-term (Other)
KW  - Modeling approach (Other)
KW  - Organ (Other)
KW  - Protocol (Other)
KW  - Self-assembled (Other)
KW  - Skin equivalents (Other)
KW  - Tissue (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:32222225
DO  - DOI:doi: 10.1016/bs.mcb.2019.11.012
UR  - https://inrepo02.dkfz.de/record/167465
ER  -