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@ARTICLE{PavezLori:167465,
      author       = {E. Pavez Loriè and P. Boukamp$^*$},
      title        = {{M}ethods in cell biology: {C}ell-derived matrices.},
      journal      = {Methods in cell biology},
      volume       = {156},
      issn         = {0091-679X},
      address      = {New York, NY [u.a.]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2021-00368},
      pages        = {309-332},
      year         = {2020},
      note         = {Volume 156, 2020, Pages 309-332#LA:A110#},
      abstract     = {Three-dimensional (3D) in vitro skin and skin cancer models
                      have become an invaluable tool in skin research. They go
                      back to 1979, when Bell and colleagues reported on the
                      establishment of a fibroblast-dependent collagen tissue
                      (Bell, Ivarsson, $\&$ Merrill, 1979). On top of such tissue
                      a stratified and differentiated epidermis could be
                      established (Bell, Merrill, $\&$ Solomon, 1979).
                      Hydrogel-based dermal equivalents have been generated ever
                      since and upon co-culture with normal human skin
                      keratinocytes, these constructs were then termed skin
                      equivalents. Due to a number of deficiencies, the most
                      important one being their restricted survival time, new
                      developments helped to circumvent premature fibroblast
                      activation and tissue destruction. By avoiding collagen for
                      the dermal equivalent (DE), we proposed, a scaffold-based
                      DE, allowing fibroblasts to reorganize the primary fibrin
                      solution into an 'authentic' dermal matrix (Boehnke et al.,
                      2007; Stark et al., 2004, 2006). With this, our goal of a
                      long-term skin equivalent-successful cultivation for several
                      months-was achieved. Nevertheless, also this model presented
                      limitations. One being its opaqueness made it difficult to
                      image the intact tissue. Another draw-back was that tumor
                      cells upon invasion used the scaffold as a guardrail leaving
                      behind an unspecific invasion pattern. All this could be
                      avoided by an approach, the fibroblast-derived matrix-based
                      model, based on the work by Ahlfors and Billiar (2007) We
                      here provide a protocol for this type of model, thereby
                      providing the basis for future work in the field of skin
                      research.},
      keywords     = {Cells, Cultured / Cytological Techniques: methods /
                      Extracellular Matrix: metabolism / Fibroblasts: cytology /
                      Fibroblasts: metabolism / Humans / Keratinocytes: cytology /
                      Keratinocytes: metabolism / 3D models (Other) / Cell-derived
                      matrix (Other) / Dermal equivalent (Other) / ECM (Other) /
                      Fibroblast (Other) / Human model (Other) / Keratinocyte
                      (Other) / Long-term (Other) / Modeling approach (Other) /
                      Organ (Other) / Protocol (Other) / Self-assembled (Other) /
                      Skin equivalents (Other) / Tissue (Other)},
      cin          = {A110},
      ddc          = {570},
      cid          = {I:(DE-He78)A110-20160331},
      pnm          = {311 - Signalling pathways, cell and tumor biology
                      (POF3-311)},
      pid          = {G:(DE-HGF)POF3-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:32222225},
      doi          = {doi: 10.1016/bs.mcb.2019.11.012},
      url          = {https://inrepo02.dkfz.de/record/167465},
}