%0 Journal Article
%A Xydia, Maria
%A Rahbari, Raheleh
%A Ruggiero, Eliana
%A Macaulay, Iain
%A Tarabichi, Maxime
%A Lohmayer, Robert
%A Wilkening, Stefan
%A Michels, Tillmann
%A Brown, Daniel
%A Vanuytven, Sebastiaan
%A Mastitskaya, Svetlana
%A Laidlaw, Sean
%A Grabe, Niels
%A Pritsch, Maria
%A Fronza, Raffaele
%A Hexel, Klaus
%A Schmitt, Steffen
%A Müller-Steinhardt, Michael
%A Halama, Niels
%A Domschke, Christoph
%A Schmidt, Manfred
%A von Kalle, Christof
%A Schütz, Florian
%A Voet, Thierry
%A Beckhove, Philipp
%T Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.
%J Nature Communications
%V 12
%N 1
%@ 2041-1723
%C [London]
%I Nature Publishing Group UK
%M DKFZ-2021-00397
%P 1119
%D 2021
%Z #EA:D015#LA:D015#
%X Regulatory CD4+ T cells (Treg) prevent tumor clearance by conventional T cells (Tconv) comprising a major obstacle of cancer immune-surveillance. Hitherto, the mechanisms of Treg repertoire formation in human cancers remain largely unclear. Here, we analyze Treg clonal origin in breast cancer patients using T-Cell Receptor and single-cell transcriptome sequencing. While Treg in peripheral blood and breast tumors are clonally distinct, Tconv clones, including tumor-antigen reactive effectors (Teff), are detected in both compartments. Tumor-infiltrating CD4+ cells accumulate into distinct transcriptome clusters, including early activated Tconv, uncommitted Teff, Th1 Teff, suppressive Treg and pro-tumorigenic Treg. Trajectory analysis suggests early activated Tconv differentiation either into Th1 Teff or into suppressive and pro-tumorigenic Treg. Importantly, Tconv, activated Tconv and Treg share highly-expanded clones contributing up to 65
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:33602930
%R 10.1038/s41467-021-21297-y
%U https://inrepo02.dkfz.de/record/167532