TY  - JOUR
AU  - Xydia, Maria
AU  - Rahbari, Raheleh
AU  - Ruggiero, Eliana
AU  - Macaulay, Iain
AU  - Tarabichi, Maxime
AU  - Lohmayer, Robert
AU  - Wilkening, Stefan
AU  - Michels, Tillmann
AU  - Brown, Daniel
AU  - Vanuytven, Sebastiaan
AU  - Mastitskaya, Svetlana
AU  - Laidlaw, Sean
AU  - Grabe, Niels
AU  - Pritsch, Maria
AU  - Fronza, Raffaele
AU  - Hexel, Klaus
AU  - Schmitt, Steffen
AU  - Müller-Steinhardt, Michael
AU  - Halama, Niels
AU  - Domschke, Christoph
AU  - Schmidt, Manfred
AU  - von Kalle, Christof
AU  - Schütz, Florian
AU  - Voet, Thierry
AU  - Beckhove, Philipp
TI  - Common clonal origin of conventional T cells and induced regulatory T cells in breast cancer patients.
JO  - Nature Communications
VL  - 12
IS  - 1
SN  - 2041-1723
CY  - [London]
PB  - Nature Publishing Group UK
M1  - DKFZ-2021-00397
SP  - 1119
PY  - 2021
N1  - #EA:D015#LA:D015#
AB  - Regulatory CD4+ T cells (Treg) prevent tumor clearance by conventional T cells (Tconv) comprising a major obstacle of cancer immune-surveillance. Hitherto, the mechanisms of Treg repertoire formation in human cancers remain largely unclear. Here, we analyze Treg clonal origin in breast cancer patients using T-Cell Receptor and single-cell transcriptome sequencing. While Treg in peripheral blood and breast tumors are clonally distinct, Tconv clones, including tumor-antigen reactive effectors (Teff), are detected in both compartments. Tumor-infiltrating CD4+ cells accumulate into distinct transcriptome clusters, including early activated Tconv, uncommitted Teff, Th1 Teff, suppressive Treg and pro-tumorigenic Treg. Trajectory analysis suggests early activated Tconv differentiation either into Th1 Teff or into suppressive and pro-tumorigenic Treg. Importantly, Tconv, activated Tconv and Treg share highly-expanded clones contributing up to 65
LB  - PUB:(DE-HGF)16
C6  - pmid:33602930
DO  - DOI:10.1038/s41467-021-21297-y
UR  - https://inrepo02.dkfz.de/record/167532
ER  -