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000167752 0247_ $$2ISSN$$a1947-5543
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000167752 041__ $$aEnglish
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000167752 1001_ $$0P:(DE-He78)ae42275b5c780352fb8f6b9e9fe70602$$aVoggenreiter, Theresa$$b0$$eFirst author$$udkfz
000167752 245__ $$aSimulation of Air Travel-Related Irradiation Exposure of Cryopreserved Mouse Germplasm Samples.
000167752 260__ $$aNew Rochelle, NY$$bLiebert$$c2021
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000167752 500__ $$a#EA:W430#LA:W430# / 319H / 2021 Aug;19(4):280-286
000167752 520__ $$aCryopreservation of genetically modified mouse lines prevents the loss of specific mutants that are of enormous scientific value for both basic and applied research. Cryopreservation of spermatozoa or preimplantation embryos enables discontinuation of breeding as well as archiving of specific lines for future studies. Regarding active inter-laboratory exchange of mutants, cryopreserved material is more advantageous to transport than live animals. However, transportation stress should not be trivialized. Security scanning of transport boxes at airports and customs, in particular, as well as additional cosmic radiation, pose a threat to undefined dosages of irradiation exposure. To simulate this, cryopreserved samples of mouse spermatozoa and preimplantation embryos were exposed to an X-ray dosage of 1 mGy in an X-ray machine. For subsequent investigation of the cell integrity of irradiated spermatozoa and embryos, spermatozoa forward motility as well as embryo developmental capacity and apoptosis values were examined and compared with nonirradiated control samples. The percentage of forward-moving spermatozoa per sample appears to be significantly reduced after irradiation exposure. The in vitro developmental capacity of preimplantation embryos as well as their relative share of apoptotic cells do not seem to be influenced by irradiation exposure. This leads to the assumption that, at least in preimplantation embryos, X-ray dosages of 1 mGy do not induce sudden severe cellular harm. Nevertheless, stochastic effects of ionizing irradiation, such as mutations, do not have a dosage threshold and always represent the potential danger of alterations to cells and cellular components, especially the DNA. This could lead to undefined mutations inducing genetic drift, in the worst case to the loss of a mutant line. We therefore strongly recommend minimizing 'transportation stress,' in particular by irradiation exposure, to keep its potential consequences in mind, and to standardize shipping procedures.
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000167752 650_7 $$2Other$$aairport security scanning
000167752 650_7 $$2Other$$acryopreservation
000167752 650_7 $$2Other$$agenetic drift
000167752 650_7 $$2Other$$agenetically modified (GM) mice
000167752 650_7 $$2Other$$alow-dose irradiation exposure
000167752 650_7 $$2Other$$ashipment
000167752 7001_ $$0P:(DE-He78)26b012bbbc85e8736a8979469a69d9f6$$aLaport, Elke$$b1$$udkfz
000167752 7001_ $$0P:(DE-He78)e14ea3ff804b628e746eece9626deffa$$aKahn-Schapowal, Barbara$$b2$$udkfz
000167752 7001_ $$0P:(DE-He78)e90646801cd6f13e7a30039429432e84$$aLang, Jens$$b3$$udkfz
000167752 7001_ $$0P:(DE-He78)1b0532fc51fd835d3bb64196e6e751fc$$aSchenkel, Johannes$$b4$$eLast author$$udkfz
000167752 773__ $$0PERI:(DE-600)2593993-2$$a10.1089/bio.2020.0046$$gp. bio.2020.0046$$n4$$p280-286$$tBiopreservation and biobanking$$v19$$x1947-5543$$y2021
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