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@ARTICLE{Voggenreiter:167752,
      author       = {T. Voggenreiter$^*$ and E. Laport$^*$ and B.
                      Kahn-Schapowal$^*$ and J. Lang$^*$ and J. Schenkel$^*$},
      title        = {{S}imulation of {A}ir {T}ravel-{R}elated {I}rradiation
                      {E}xposure of {C}ryopreserved {M}ouse {G}ermplasm
                      {S}amples.},
      journal      = {Biopreservation and biobanking},
      volume       = {19},
      number       = {4},
      issn         = {1947-5543},
      address      = {New Rochelle, NY},
      publisher    = {Liebert},
      reportid     = {DKFZ-2021-00516},
      pages        = {280-286},
      year         = {2021},
      note         = {#EA:W430#LA:W430# / 319H / 2021 Aug;19(4):280-286},
      abstract     = {Cryopreservation of genetically modified mouse lines
                      prevents the loss of specific mutants that are of enormous
                      scientific value for both basic and applied research.
                      Cryopreservation of spermatozoa or preimplantation embryos
                      enables discontinuation of breeding as well as archiving of
                      specific lines for future studies. Regarding active
                      inter-laboratory exchange of mutants, cryopreserved material
                      is more advantageous to transport than live animals.
                      However, transportation stress should not be trivialized.
                      Security scanning of transport boxes at airports and
                      customs, in particular, as well as additional cosmic
                      radiation, pose a threat to undefined dosages of irradiation
                      exposure. To simulate this, cryopreserved samples of mouse
                      spermatozoa and preimplantation embryos were exposed to an
                      X-ray dosage of 1 mGy in an X-ray machine. For subsequent
                      investigation of the cell integrity of irradiated
                      spermatozoa and embryos, spermatozoa forward motility as
                      well as embryo developmental capacity and apoptosis values
                      were examined and compared with nonirradiated control
                      samples. The percentage of forward-moving spermatozoa per
                      sample appears to be significantly reduced after irradiation
                      exposure. The in vitro developmental capacity of
                      preimplantation embryos as well as their relative share of
                      apoptotic cells do not seem to be influenced by irradiation
                      exposure. This leads to the assumption that, at least in
                      preimplantation embryos, X-ray dosages of 1 mGy do not
                      induce sudden severe cellular harm. Nevertheless, stochastic
                      effects of ionizing irradiation, such as mutations, do not
                      have a dosage threshold and always represent the potential
                      danger of alterations to cells and cellular components,
                      especially the DNA. This could lead to undefined mutations
                      inducing genetic drift, in the worst case to the loss of a
                      mutant line. We therefore strongly recommend minimizing
                      'transportation stress,' in particular by irradiation
                      exposure, to keep its potential consequences in mind, and to
                      standardize shipping procedures.},
      keywords     = {airport security scanning (Other) / cryopreservation
                      (Other) / genetic drift (Other) / genetically modified (GM)
                      mice (Other) / low-dose irradiation exposure (Other) /
                      shipment (Other)},
      cin          = {W430 / W060},
      ddc          = {610},
      cid          = {I:(DE-He78)W430-20160331 / I:(DE-He78)W060-20160331},
      pnm          = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
      pid          = {G:(DE-HGF)POF4-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33646019},
      doi          = {10.1089/bio.2020.0046},
      url          = {https://inrepo02.dkfz.de/record/167752},
}