%0 Journal Article
%A Latif, Sara
%A Gottschamel, Johanna
%A Syed, Tahira
%A Younus, Iqra
%A Gull, Kehkshan
%A Sameeullah, Muhammad
%A Batool, Neelam
%A Lössl, Andreas Günter
%A Mariz, Filipe
%A Müller, Martin
%A Mirza, Bushra
%A Waheed, Mohammad Tahir
%T Inducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen.
%J Biotechnology and applied biochemistry
%V 69
%N 2
%@ 1470-8744
%C Hoboken, NJ [u.a.]
%I Wiley
%M DKFZ-2021-00522
%P 596-611
%D 2022
%Z 2022 Apr;69(2):596-611
%X Human papillomavirus type 16 (HPV-16) is the major HPV type involved in causing cervical cancer among women. The disease burden is high in developing and underdeveloped countries. Previously, the constitutive expression of HPV-16 L1 protein led to male sterility in transplastomic tobacco plants. Here, the HPV-16 L1 gene was expressed in chloroplasts of Nicotiana tabacum under the control of an ethanol-inducible promoter, trans-activated by nucleus-derived signal peptide. Plants containing nuclear component were transformed with transformation vector pEXP-T7-L1 by biolistic gun. The transformation and homoplasmic status of transformed plants was verified by polymerase chain reaction (PCR) and Southern blotting, respectively. Protein was induced by spraying 5
%K Biopharmaceuticals (Other)
%K L1 gene (Other)
%K capsomeres (Other)
%K cervical cancer (Other)
%K ethanol-inducible system (Other)
%K human papillomavirus (Other)
%K plant-based vaccines (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:33650709
%R 10.1002/bab.2136
%U https://inrepo02.dkfz.de/record/167758