000167758 001__ 167758
000167758 005__ 20240229133547.0
000167758 0247_ $$2doi$$a10.1002/bab.2136
000167758 0247_ $$2pmid$$apmid:33650709
000167758 0247_ $$2ISSN$$a0885-4513
000167758 0247_ $$2ISSN$$a1470-8744
000167758 0247_ $$2altmetric$$aaltmetric:101195851
000167758 037__ $$aDKFZ-2021-00522
000167758 041__ $$aEnglish
000167758 082__ $$a660
000167758 1001_ $$aLatif, Sara$$b0
000167758 245__ $$aInducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen.
000167758 260__ $$aHoboken, NJ [u.a.]$$bWiley$$c2022
000167758 3367_ $$2DRIVER$$aarticle
000167758 3367_ $$2DataCite$$aOutput Types/Journal article
000167758 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1651056780_21832
000167758 3367_ $$2BibTeX$$aARTICLE
000167758 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000167758 3367_ $$00$$2EndNote$$aJournal Article
000167758 500__ $$a2022 Apr;69(2):596-611
000167758 520__ $$aHuman papillomavirus type 16 (HPV-16) is the major HPV type involved in causing cervical cancer among women. The disease burden is high in developing and underdeveloped countries. Previously, the constitutive expression of HPV-16 L1 protein led to male sterility in transplastomic tobacco plants. Here, the HPV-16 L1 gene was expressed in chloroplasts of Nicotiana tabacum under the control of an ethanol-inducible promoter, trans-activated by nucleus-derived signal peptide. Plants containing nuclear component were transformed with transformation vector pEXP-T7-L1 by biolistic gun. The transformation and homoplasmic status of transformed plants was verified by polymerase chain reaction (PCR) and Southern blotting, respectively. Protein was induced by spraying 5% ethanol for seven consecutive days. The correct folding of L1 protein was confirmed by antigen-capture ELISA using a conformation-specific antibody. The L1 protein accumulated up to 3 μg/gm of fresh plant material. The L1 protein was further purified using affinity chromatography. All transplastomic plants developed normal flowers and produced viable seeds upon self-pollination. Pollens also showed completely normal structure under light microscope and scanning electron microscopy. This data confirms the use of the inducible expression as plant-safe approach for expressing transgenes in plants, especially those genes that cause detrimental effects on plant growth and morphology. This article is protected by copyright. All rights reserved.
000167758 536__ $$0G:(DE-HGF)POF4-316$$a316 - Infektionen, Entzündung und Krebs (POF4-316)$$cPOF4-316$$fPOF IV$$x0
000167758 588__ $$aDataset connected to CrossRef, PubMed,
000167758 650_7 $$2Other$$aBiopharmaceuticals
000167758 650_7 $$2Other$$aL1 gene
000167758 650_7 $$2Other$$acapsomeres
000167758 650_7 $$2Other$$acervical cancer
000167758 650_7 $$2Other$$aethanol-inducible system
000167758 650_7 $$2Other$$ahuman papillomavirus
000167758 650_7 $$2Other$$aplant-based vaccines
000167758 7001_ $$aGottschamel, Johanna$$b1
000167758 7001_ $$aSyed, Tahira$$b2
000167758 7001_ $$aYounus, Iqra$$b3
000167758 7001_ $$aGull, Kehkshan$$b4
000167758 7001_ $$aSameeullah, Muhammad$$b5
000167758 7001_ $$aBatool, Neelam$$b6
000167758 7001_ $$aLössl, Andreas Günter$$b7
000167758 7001_ $$0P:(DE-HGF)0$$aMariz, Filipe$$b8
000167758 7001_ $$0P:(DE-He78)47d2c5b8ee00ab8a0c16e14137427579$$aMüller, Martin$$b9$$udkfz
000167758 7001_ $$aMirza, Bushra$$b10
000167758 7001_ $$00000-0002-9861-462X$$aWaheed, Mohammad Tahir$$b11
000167758 773__ $$0PERI:(DE-600)1496341-3$$a10.1002/bab.2136$$gp. bab.2136$$n2$$p596-611$$tBiotechnology and applied biochemistry$$v69$$x1470-8744$$y2022
000167758 909CO $$ooai:inrepo02.dkfz.de:167758$$pVDB
000167758 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-HGF)0$$aDeutsches Krebsforschungszentrum$$b8$$kDKFZ
000167758 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)47d2c5b8ee00ab8a0c16e14137427579$$aDeutsches Krebsforschungszentrum$$b9$$kDKFZ
000167758 9131_ $$0G:(DE-HGF)POF4-316$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vInfektionen, Entzündung und Krebs$$x0
000167758 9130_ $$0G:(DE-HGF)POF3-316$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vInfections and cancer$$x0
000167758 9141_ $$y2021
000167758 915__ $$0StatID:(DE-HGF)3001$$2StatID$$aDEAL Wiley$$d2021-01-28$$wger
000167758 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-01-28
000167758 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-01-28
000167758 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-01-28
000167758 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)1060$$2StatID$$aDBCoverage$$bCurrent Contents - Agriculture, Biology and Environmental Sciences$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bBIOTECHNOL APPL BIOC : 2021$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2022-11-15
000167758 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5$$d2022-11-15
000167758 9201_ $$0I:(DE-He78)F035-20160331$$kF035$$lF035 Tumorvirus-spez. Vakzinierungsstrategie$$x0
000167758 980__ $$ajournal
000167758 980__ $$aVDB
000167758 980__ $$aI:(DE-He78)F035-20160331
000167758 980__ $$aUNRESTRICTED