TY  - JOUR
AU  - Latif, Sara
AU  - Gottschamel, Johanna
AU  - Syed, Tahira
AU  - Younus, Iqra
AU  - Gull, Kehkshan
AU  - Sameeullah, Muhammad
AU  - Batool, Neelam
AU  - Lössl, Andreas Günter
AU  - Mariz, Filipe
AU  - Müller, Martin
AU  - Mirza, Bushra
AU  - Waheed, Mohammad Tahir
TI  - Inducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen.
JO  - Biotechnology and applied biochemistry
VL  - 69
IS  - 2
SN  - 1470-8744
CY  - Hoboken, NJ [u.a.]
PB  - Wiley
M1  - DKFZ-2021-00522
SP  - 596-611
PY  - 2022
N1  - 2022 Apr;69(2):596-611
AB  - Human papillomavirus type 16 (HPV-16) is the major HPV type involved in causing cervical cancer among women. The disease burden is high in developing and underdeveloped countries. Previously, the constitutive expression of HPV-16 L1 protein led to male sterility in transplastomic tobacco plants. Here, the HPV-16 L1 gene was expressed in chloroplasts of Nicotiana tabacum under the control of an ethanol-inducible promoter, trans-activated by nucleus-derived signal peptide. Plants containing nuclear component were transformed with transformation vector pEXP-T7-L1 by biolistic gun. The transformation and homoplasmic status of transformed plants was verified by polymerase chain reaction (PCR) and Southern blotting, respectively. Protein was induced by spraying 5
KW  - Biopharmaceuticals (Other)
KW  - L1 gene (Other)
KW  - capsomeres (Other)
KW  - cervical cancer (Other)
KW  - ethanol-inducible system (Other)
KW  - human papillomavirus (Other)
KW  - plant-based vaccines (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33650709
DO  - DOI:10.1002/bab.2136
UR  - https://inrepo02.dkfz.de/record/167758
ER  -