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@ARTICLE{Latif:167758,
      author       = {S. Latif and J. Gottschamel and T. Syed and I. Younus and
                      K. Gull and M. Sameeullah and N. Batool and A. G. Lössl and
                      F. Mariz$^*$ and M. Müller$^*$ and B. Mirza and M. T.
                      Waheed},
      title        = {{I}nducible expression of human papillomavirus-16 {L}1
                      capsomeres in the plastomes of {N}icotiana tabacum:
                      {T}ransplastomic plants develop normal flowers and pollen.},
      journal      = {Biotechnology and applied biochemistry},
      volume       = {69},
      number       = {2},
      issn         = {1470-8744},
      address      = {Hoboken, NJ [u.a.]},
      publisher    = {Wiley},
      reportid     = {DKFZ-2021-00522},
      pages        = {596-611},
      year         = {2022},
      note         = {2022 Apr;69(2):596-611},
      abstract     = {Human papillomavirus type 16 (HPV-16) is the major HPV type
                      involved in causing cervical cancer among women. The disease
                      burden is high in developing and underdeveloped countries.
                      Previously, the constitutive expression of HPV-16 L1 protein
                      led to male sterility in transplastomic tobacco plants.
                      Here, the HPV-16 L1 gene was expressed in chloroplasts of
                      Nicotiana tabacum under the control of an ethanol-inducible
                      promoter, trans-activated by nucleus-derived signal peptide.
                      Plants containing nuclear component were transformed with
                      transformation vector pEXP-T7-L1 by biolistic gun. The
                      transformation and homoplasmic status of transformed plants
                      was verified by polymerase chain reaction (PCR) and Southern
                      blotting, respectively. Protein was induced by spraying
                      $5\%$ ethanol for seven consecutive days. The correct
                      folding of L1 protein was confirmed by antigen-capture ELISA
                      using a conformation-specific antibody. The L1 protein
                      accumulated up to 3 μg/gm of fresh plant material. The L1
                      protein was further purified using affinity chromatography.
                      All transplastomic plants developed normal flowers and
                      produced viable seeds upon self-pollination. Pollens also
                      showed completely normal structure under light microscope
                      and scanning electron microscopy. This data confirms the use
                      of the inducible expression as plant-safe approach for
                      expressing transgenes in plants, especially those genes that
                      cause detrimental effects on plant growth and morphology.
                      This article is protected by copyright. All rights
                      reserved.},
      keywords     = {Biopharmaceuticals (Other) / L1 gene (Other) / capsomeres
                      (Other) / cervical cancer (Other) / ethanol-inducible system
                      (Other) / human papillomavirus (Other) / plant-based
                      vaccines (Other)},
      cin          = {F035},
      ddc          = {660},
      cid          = {I:(DE-He78)F035-20160331},
      pnm          = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
      pid          = {G:(DE-HGF)POF4-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33650709},
      doi          = {10.1002/bab.2136},
      url          = {https://inrepo02.dkfz.de/record/167758},
}