Home > Publications database > Inducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen. > print

001     167758
005     20240229133547.0
024 7 _ |a 10.1002/bab.2136
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024 7 _ |a 0885-4513
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024 7 _ |a 1470-8744
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037 _ _ |a DKFZ-2021-00522
041 _ _ |a English
082 _ _ |a 660
100 1 _ |a Latif, Sara
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245 _ _ |a Inducible expression of human papillomavirus-16 L1 capsomeres in the plastomes of Nicotiana tabacum: Transplastomic plants develop normal flowers and pollen.
260 _ _ |a Hoboken, NJ [u.a.]
|c 2022
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336 7 _ |a article
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500 _ _ |a 2022 Apr;69(2):596-611
520 _ _ |a Human papillomavirus type 16 (HPV-16) is the major HPV type involved in causing cervical cancer among women. The disease burden is high in developing and underdeveloped countries. Previously, the constitutive expression of HPV-16 L1 protein led to male sterility in transplastomic tobacco plants. Here, the HPV-16 L1 gene was expressed in chloroplasts of Nicotiana tabacum under the control of an ethanol-inducible promoter, trans-activated by nucleus-derived signal peptide. Plants containing nuclear component were transformed with transformation vector pEXP-T7-L1 by biolistic gun. The transformation and homoplasmic status of transformed plants was verified by polymerase chain reaction (PCR) and Southern blotting, respectively. Protein was induced by spraying 5% ethanol for seven consecutive days. The correct folding of L1 protein was confirmed by antigen-capture ELISA using a conformation-specific antibody. The L1 protein accumulated up to 3 μg/gm of fresh plant material. The L1 protein was further purified using affinity chromatography. All transplastomic plants developed normal flowers and produced viable seeds upon self-pollination. Pollens also showed completely normal structure under light microscope and scanning electron microscopy. This data confirms the use of the inducible expression as plant-safe approach for expressing transgenes in plants, especially those genes that cause detrimental effects on plant growth and morphology. This article is protected by copyright. All rights reserved.
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650 _ 7 |a Biopharmaceuticals
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650 _ 7 |a L1 gene
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650 _ 7 |a capsomeres
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650 _ 7 |a cervical cancer
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650 _ 7 |a ethanol-inducible system
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650 _ 7 |a human papillomavirus
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650 _ 7 |a plant-based vaccines
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700 1 _ |a Gottschamel, Johanna
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700 1 _ |a Syed, Tahira
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700 1 _ |a Younus, Iqra
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700 1 _ |a Gull, Kehkshan
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700 1 _ |a Sameeullah, Muhammad
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700 1 _ |a Batool, Neelam
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700 1 _ |a Lössl, Andreas Günter
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700 1 _ |a Mariz, Filipe
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700 1 _ |a Müller, Martin
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700 1 _ |a Mirza, Bushra
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700 1 _ |a Waheed, Mohammad Tahir
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773 _ _ |a 10.1002/bab.2136
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