TY  - JOUR
AU  - Sadoun, Anaïs
AU  - Biarnes-Pelicot, Martine
AU  - Ghesquiere-Dierickx, Laura
AU  - Wu, Ambroise
AU  - Théodoly, Olivier
AU  - Limozin, Laurent
AU  - Hamon, Yannick
AU  - Puech, Pierre-Henri
TI  - Controlling T cells spreading, mechanics and activation by micropatterning.
JO  - Scientific reports
VL  - 11
IS  - 1
SN  - 2045-2322
CY  - [London]
PB  - Macmillan Publishers Limited, part of Springer Nature
M1  - DKFZ-2021-00724
SP  - 6783
PY  - 2021
AB  - We designed a strategy, based on a careful examination of the activation capabilities of proteins and antibodies used as substrates for adhering T cells, coupled to protein microstamping to control at the same time the position, shape, spreading, mechanics and activation state of T cells. Once adhered on patterns, we examined the capacities of T cells to be activated with soluble anti CD3, in comparison to T cells adhered to a continuously decorated substrate with the same density of ligands. We show that, in our hand, adhering onto an anti CD45 antibody decorated surface was not affecting T cell calcium fluxes, even adhered on variable size micro-patterns. Aside, we analyzed the T cell mechanics, when spread on pattern or not, using Atomic Force Microscopy indentation. By expressing MEGF10 as a non immune adhesion receptor in T cells we measured the very same spreading area on PLL substrates and Young modulus than non modified cells, immobilized on anti CD45 antibodies, while retaining similar activation capabilities using soluble anti CD3 antibodies or through model APC contacts. We propose that our system is a way to test activation or anergy of T cells with defined adhesion and mechanical characteristics, and may allow to dissect fine details of these mechanisms since it allows to observe homogenized populations in standardized T cell activation assays.
LB  - PUB:(DE-HGF)16
C6  - pmid:33762632
C2  - pmc:PMC7991639
DO  - DOI:10.1038/s41598-021-86133-1
UR  - https://inrepo02.dkfz.de/record/168184
ER  -