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@ARTICLE{Sadoun:168184,
      author       = {A. Sadoun and M. Biarnes-Pelicot and L.
                      Ghesquiere-Dierickx$^*$ and A. Wu and O. Théodoly and L.
                      Limozin and Y. Hamon and P.-H. Puech},
      title        = {{C}ontrolling {T} cells spreading, mechanics and activation
                      by micropatterning.},
      journal      = {Scientific reports},
      volume       = {11},
      number       = {1},
      issn         = {2045-2322},
      address      = {[London]},
      publisher    = {Macmillan Publishers Limited, part of Springer Nature},
      reportid     = {DKFZ-2021-00724},
      pages        = {6783},
      year         = {2021},
      abstract     = {We designed a strategy, based on a careful examination of
                      the activation capabilities of proteins and antibodies used
                      as substrates for adhering T cells, coupled to protein
                      microstamping to control at the same time the position,
                      shape, spreading, mechanics and activation state of T cells.
                      Once adhered on patterns, we examined the capacities of T
                      cells to be activated with soluble anti CD3, in comparison
                      to T cells adhered to a continuously decorated substrate
                      with the same density of ligands. We show that, in our hand,
                      adhering onto an anti CD45 antibody decorated surface was
                      not affecting T cell calcium fluxes, even adhered on
                      variable size micro-patterns. Aside, we analyzed the T cell
                      mechanics, when spread on pattern or not, using Atomic Force
                      Microscopy indentation. By expressing MEGF10 as a non immune
                      adhesion receptor in T cells we measured the very same
                      spreading area on PLL substrates and Young modulus than non
                      modified cells, immobilized on anti CD45 antibodies, while
                      retaining similar activation capabilities using soluble anti
                      CD3 antibodies or through model APC contacts. We propose
                      that our system is a way to test activation or anergy of T
                      cells with defined adhesion and mechanical characteristics,
                      and may allow to dissect fine details of these mechanisms
                      since it allows to observe homogenized populations in
                      standardized T cell activation assays.},
      cin          = {E040},
      ddc          = {600},
      cid          = {I:(DE-He78)E040-20160331},
      pnm          = {315 - Bildgebung und Radioonkologie (POF4-315)},
      pid          = {G:(DE-HGF)POF4-315},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33762632},
      pmc          = {pmc:PMC7991639},
      doi          = {10.1038/s41598-021-86133-1},
      url          = {https://inrepo02.dkfz.de/record/168184},
}