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000168290 0247_ $$2doi$$a10.1016/j.mimet.2021.106150
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000168290 0247_ $$2ISSN$$a0167-7012
000168290 0247_ $$2ISSN$$a1872-8359
000168290 037__ $$aDKFZ-2021-00801
000168290 041__ $$aEnglish
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000168290 1001_ $$aBenga, Laurentiu$$b0
000168290 245__ $$aDifferentiation among the most important Rodentibacter species by multiplex PCR assays targeting the ITSile+ala sequences of the rRNA operons.
000168290 260__ $$aNew York, NY$$bElsevier$$c2021
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000168290 520__ $$aScreening for the Rodentibacter species is part of the microbiologic quality assurance programs of laboratory rodents all over the world. Nevertheless, currently there are no PCR amplification techniques available for the diagnostic of R. ratti, R. heidelbergensis and of a Rodentibacter related β-haemolytic taxon. The aim of this study was to utilize the differences in the sequence of the Internal Transcribed Spacer (ITS) regions of R. pneumotropicus, R. heylii, R. ratti, R. heidelbergensis and of the β-haemolytic Rodentibacter taxon for the design of specific PCR assays for these species. The ITSile+ala sequence variations allowed the design of specific forward and reverse primers for each species included, that could be combined in different multiplex assays. The performance characteristics specificity and sensitivity registered for each primer pair against a diverse collection of Pasteurellaceae isolated from rats and mice and of further non-Pasteurellaceae strains was 100% for all five Rodentibacter species included. In addition, the PCR assays displayed high limits of detection and could be successfully used for detection of Rodentibacter spp. DNA in clinical swabs of laboratory mice and rats. Overall, the assays described here represent the first PCRs able to diagnose R. ratti, R. heidelbergensis and the β-haemolytic Rodentibacter taxon, whose diagnostic to species level could further facilitate better understanding of their geographic distribution, prevalence, and biology in the future.
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000168290 650_7 $$2Other$$aInternal transcribed spacer
000168290 650_7 $$2Other$$aPCR
000168290 650_7 $$2Other$$aRodentibacter
000168290 650_7 $$2Other$$aRodents
000168290 7001_ $$aEngelhardt, Eva$$b1
000168290 7001_ $$aBenten, W Peter M$$b2
000168290 7001_ $$0P:(DE-He78)3e12f398defbd6acd71fb1664090db10$$aNicklas, Werner$$b3$$udkfz
000168290 7001_ $$aSager, Martin$$b4
000168290 773__ $$0PERI:(DE-600)1483012-7$$a10.1016/j.mimet.2021.106150$$gVol. 182, p. 106150 -$$p106150$$tJournal of microbiological methods$$v182$$x0167-7012$$y2021
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