TY  - JOUR
AU  - Benga, Laurentiu
AU  - Engelhardt, Eva
AU  - Benten, W Peter M
AU  - Nicklas, Werner
AU  - Sager, Martin
TI  - Differentiation among the most important Rodentibacter species by multiplex PCR assays targeting the ITSile+ala sequences of the rRNA operons.
JO  - Journal of microbiological methods
VL  - 182
SN  - 0167-7012
CY  - New York, NY
PB  - Elsevier
M1  - DKFZ-2021-00801
SP  - 106150
PY  - 2021
AB  - Screening for the Rodentibacter species is part of the microbiologic quality assurance programs of laboratory rodents all over the world. Nevertheless, currently there are no PCR amplification techniques available for the diagnostic of R. ratti, R. heidelbergensis and of a Rodentibacter related β-haemolytic taxon. The aim of this study was to utilize the differences in the sequence of the Internal Transcribed Spacer (ITS) regions of R. pneumotropicus, R. heylii, R. ratti, R. heidelbergensis and of the β-haemolytic Rodentibacter taxon for the design of specific PCR assays for these species. The ITSile+ala sequence variations allowed the design of specific forward and reverse primers for each species included, that could be combined in different multiplex assays. The performance characteristics specificity and sensitivity registered for each primer pair against a diverse collection of Pasteurellaceae isolated from rats and mice and of further non-Pasteurellaceae strains was 100
KW  - Internal transcribed spacer (Other)
KW  - PCR (Other)
KW  - Rodentibacter (Other)
KW  - Rodents (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33503485
DO  - DOI:10.1016/j.mimet.2021.106150
UR  - https://inrepo02.dkfz.de/record/168290
ER  -