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@ARTICLE{Benga:168290,
      author       = {L. Benga and E. Engelhardt and W. P. M. Benten and W.
                      Nicklas$^*$ and M. Sager},
      title        = {{D}ifferentiation among the most important {R}odentibacter
                      species by multiplex {PCR} assays targeting the {ITS}ile+ala
                      sequences of the r{RNA} operons.},
      journal      = {Journal of microbiological methods},
      volume       = {182},
      issn         = {0167-7012},
      address      = {New York, NY},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2021-00801},
      pages        = {106150},
      year         = {2021},
      abstract     = {Screening for the Rodentibacter species is part of the
                      microbiologic quality assurance programs of laboratory
                      rodents all over the world. Nevertheless, currently there
                      are no PCR amplification techniques available for the
                      diagnostic of R. ratti, R. heidelbergensis and of a
                      Rodentibacter related β-haemolytic taxon. The aim of this
                      study was to utilize the differences in the sequence of the
                      Internal Transcribed Spacer (ITS) regions of R.
                      pneumotropicus, R. heylii, R. ratti, R. heidelbergensis and
                      of the β-haemolytic Rodentibacter taxon for the design of
                      specific PCR assays for these species. The ITSile+ala
                      sequence variations allowed the design of specific forward
                      and reverse primers for each species included, that could be
                      combined in different multiplex assays. The performance
                      characteristics specificity and sensitivity registered for
                      each primer pair against a diverse collection of
                      Pasteurellaceae isolated from rats and mice and of further
                      non-Pasteurellaceae strains was $100\%$ for all five
                      Rodentibacter species included. In addition, the PCR assays
                      displayed high limits of detection and could be successfully
                      used for detection of Rodentibacter spp. DNA in clinical
                      swabs of laboratory mice and rats. Overall, the assays
                      described here represent the first PCRs able to diagnose R.
                      ratti, R. heidelbergensis and the β-haemolytic
                      Rodentibacter taxon, whose diagnostic to species level could
                      further facilitate better understanding of their geographic
                      distribution, prevalence, and biology in the future.},
      keywords     = {Internal transcribed spacer (Other) / PCR (Other) /
                      Rodentibacter (Other) / Rodents (Other)},
      cin          = {W440},
      ddc          = {570},
      cid          = {I:(DE-He78)W440-20160331},
      pnm          = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
      pid          = {G:(DE-HGF)POF4-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:33503485},
      doi          = {10.1016/j.mimet.2021.106150},
      url          = {https://inrepo02.dkfz.de/record/168290},
}