%0 Journal Article
%A Butt, Julia Anna
%A Murugan, Rajagopal
%A Hippchen, Theresa
%A Olberg, Sylvia
%A van Straaten, Monique
%A Wardemann, Hedda
%A Stebbins, Erec
%A Kräusslich, Hans-Georg
%A Bartenschlager, Ralf
%A Brenner, Hermann
%A Laketa, Vibor
%A Schöttker, Ben
%A Müller, Barbara
%A Merle, Uta
%A Waterboer, Tim
%T From Multiplex Serology to Serolomics-A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome.
%J Viruses
%V 13
%N 5
%@ 1999-4915
%C Basel
%I MDPI
%M DKFZ-2021-00994
%P 749
%D 2021
%Z #EA:F022#LA:F022#
%X The emerging SARS-CoV-2 pandemic entails an urgent need for specific and sensitive high-throughput serological assays to assess SARS-CoV-2 epidemiology. We, therefore, aimed at developing a fluorescent-bead based SARS-CoV-2 multiplex serology assay for detection of antibody responses to the SARS-CoV-2 proteome. Proteins of the SARS-CoV-2 proteome and protein N of SARS-CoV-1 and common cold Coronaviruses (ccCoVs) were recombinantly expressed in E. coli or HEK293 cells. Assay performance was assessed in a COVID-19 case cohort (n = 48 hospitalized patients from Heidelberg) as well as n = 85 age- and sex-matched pre-pandemic controls from the ESTHER study. Assay validation included comparison with home-made immunofluorescence and commercial enzyme-linked immunosorbent (ELISA) assays. A sensitivity of 100
%K SARS-CoV-2 (Other)
%K multiplex serology (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:33923338
%R 10.3390/v13050749
%U https://inrepo02.dkfz.de/record/168686