TY  - JOUR
AU  - Butt, Julia Anna
AU  - Murugan, Rajagopal
AU  - Hippchen, Theresa
AU  - Olberg, Sylvia
AU  - van Straaten, Monique
AU  - Wardemann, Hedda
AU  - Stebbins, Erec
AU  - Kräusslich, Hans-Georg
AU  - Bartenschlager, Ralf
AU  - Brenner, Hermann
AU  - Laketa, Vibor
AU  - Schöttker, Ben
AU  - Müller, Barbara
AU  - Merle, Uta
AU  - Waterboer, Tim
TI  - From Multiplex Serology to Serolomics-A Novel Approach to the Antibody Response against the SARS-CoV-2 Proteome.
JO  - Viruses
VL  - 13
IS  - 5
SN  - 1999-4915
CY  - Basel
PB  - MDPI
M1  - DKFZ-2021-00994
SP  - 749
PY  - 2021
N1  - #EA:F022#LA:F022#
AB  - The emerging SARS-CoV-2 pandemic entails an urgent need for specific and sensitive high-throughput serological assays to assess SARS-CoV-2 epidemiology. We, therefore, aimed at developing a fluorescent-bead based SARS-CoV-2 multiplex serology assay for detection of antibody responses to the SARS-CoV-2 proteome. Proteins of the SARS-CoV-2 proteome and protein N of SARS-CoV-1 and common cold Coronaviruses (ccCoVs) were recombinantly expressed in E. coli or HEK293 cells. Assay performance was assessed in a COVID-19 case cohort (n = 48 hospitalized patients from Heidelberg) as well as n = 85 age- and sex-matched pre-pandemic controls from the ESTHER study. Assay validation included comparison with home-made immunofluorescence and commercial enzyme-linked immunosorbent (ELISA) assays. A sensitivity of 100
KW  - SARS-CoV-2 (Other)
KW  - multiplex serology (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:33923338
DO  - DOI:10.3390/v13050749
UR  - https://inrepo02.dkfz.de/record/168686
ER  -