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@ARTICLE{Inverso:168968,
      author       = {D. Inverso$^*$ and J. Shi$^*$ and K. H. Lee$^*$ and M.
                      Jakab$^*$ and S. Ben-Moshe and S. R. Kulkarni$^*$ and M.
                      Schneider$^*$ and G. Wang$^*$ and M. Komeili and P. A.
                      Vélez$^*$ and M. Riedel$^*$ and C. Spegg$^*$ and T. Ruppert
                      and C. Schaeffer-Reiss and D. Helm$^*$ and I. Singh$^*$ and
                      M. Boutros$^*$ and S. Chintharlapalli and M.
                      Heikenwalder$^*$ and S. Itzkovitz and H. Augustin$^*$},
      title        = {{A} spatial vascular transcriptomic, proteomic, and
                      phosphoproteomic atlas unveils an angiocrine {T}ie-{W}nt
                      signaling axis in the liver.},
      journal      = {Developmental cell},
      volume       = {56},
      number       = {11},
      issn         = {1534-5807},
      address      = {New York, NY},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2021-01164},
      pages        = {1677-1693.e10},
      year         = {2021},
      note         = {#EA:A190#LA:A190#/2021 Jun 7;56(11):1677-1693.e10},
      abstract     = {Single-cell transcriptomics (scRNA-seq) has revolutionized
                      the understanding of the spatial architecture of tissue
                      structure and function. Advancing the 'transcript-centric'
                      view of scRNA-seq analyses is presently restricted by the
                      limited resolution of proteomics and genome-wide techniques
                      to analyze post-translational modifications. Here, by
                      combining spatial cell sorting with transcriptomics and
                      quantitative proteomics/phosphoproteomics, we established
                      the spatially resolved proteome landscape of the liver
                      endothelium, yielding deep mechanistic insight into zonated
                      vascular signaling mechanisms. Phosphorylation of receptor
                      tyrosine kinases was detected preferentially in the central
                      vein area, resulting in an atypical enrichment of tyrosine
                      phosphorylation. Prototypic biological validation identified
                      Tie receptor signaling as a selective and specific regulator
                      of vascular Wnt activity orchestrating angiocrine signaling,
                      thereby controlling hepatocyte function during liver
                      regeneration. Taken together, the study has yielded
                      fundamental insight into the spatial organization of liver
                      endothelial cell signaling. Spatial sorting may be employed
                      as a universally adaptable strategy for multiomic analyses
                      of scRNA-seq-defined cellular (sub)-populations.},
      keywords     = {Tie1 (Other) / Tie2 (Other) / Wnt (Other) / angiocrine
                      factors (Other) / liver endothelial cell (L-EC) (Other) /
                      phosphoproteomics (Other) / proteomics (Other) /
                      transcriptomics (Other) / vascular zonation (Other)},
      cin          = {A190 / W120 / F180 / B110},
      ddc          = {610},
      cid          = {I:(DE-He78)A190-20160331 / I:(DE-He78)W120-20160331 /
                      I:(DE-He78)F180-20160331 / I:(DE-He78)B110-20160331},
      pnm          = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
      pid          = {G:(DE-HGF)POF4-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34038707},
      doi          = {10.1016/j.devcel.2021.05.001},
      url          = {https://inrepo02.dkfz.de/record/168968},
}