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@ARTICLE{Sauter:169025,
      author       = {M. Sauter and K. I. Foerster and J. Benzel$^*$ and S.
                      Pfister$^*$ and K. W. Pajtler$^*$ and W. E. Haefeli and J.
                      Burhenne},
      title        = {{B}ioanalysis of selinexor in mouse plasma micro-samples
                      utilizing {UPLC}-{MS}/{MS}.},
      journal      = {Journal of chromatography / B},
      volume       = {1176},
      issn         = {1570-0232},
      address      = {New York, NY [u.a.]},
      publisher    = {Science Direct},
      reportid     = {DKFZ-2021-01193},
      pages        = {122781},
      year         = {2021},
      abstract     = {Selinexor, a first-in-class inhibitor of the nuclear export
                      protein Exportin-1 (XPO1), was recently approved for the
                      treatment of multiple myeloma in combination with
                      dexamethasone, and as monotherapy for diffuse large B-cell
                      lymphoma. To enable investigations of selinexor in mice, we
                      established and validated an ultrahigh-performance liquid
                      chromatography - tandem mass spectrometry (UPLC-MS/MS) assay
                      in the plasma concentration range of 1-1000 ng/mL using
                      plasma microsamples of 5 µL. Protein depletion with
                      acetonitrile was used for efficient isolation of selinexor
                      which was followed by a dilution step, resulting in a
                      scalable sample processing. Quantification was performed
                      with positive electrospray ionization tandem mass
                      spectrometry in the selected reaction monitoring mode. Due
                      to the high sensitivity of the quantification and the
                      scalable sample processing procedure, the assay can be used
                      for different concentration ranges to either further
                      decrease the achievable lower limit of quantification or to
                      reduce the amount of plasma used. The assay showed interday
                      and intraday accuracy of $89.0-109.0\%$ with a corresponding
                      $precision ≤ 14.1\%.$ Suitability for investigations of
                      selinexor in small animal experiments was demonstrated by
                      determination of plasma selinexor in mice after oral
                      administration.},
      keywords     = {Bioanalysis (Other) / Pharmacokinetics (Other) / Selinexor
                      (Other) / Tandem Mass Spectrometry (Other) / UPLC (Other)},
      cin          = {B062 / HD01},
      ddc          = {540},
      cid          = {I:(DE-He78)B062-20160331 / I:(DE-He78)HD01-20160331},
      pnm          = {312 - Funktionelle und strukturelle Genomforschung
                      (POF4-312)},
      pid          = {G:(DE-HGF)POF4-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34051651},
      doi          = {10.1016/j.jchromb.2021.122781},
      url          = {https://inrepo02.dkfz.de/record/169025},
}