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@ARTICLE{Montassier:169733,
author = {E. Montassier and R. Valdés-Mas and E. Batard and N. Zmora
and M. Dori-Bachash and J. Suez and E. Elinav$^*$},
title = {{P}robiotics impact the antibiotic resistance gene
reservoir along the human {GI} tract in a person-specific
and antibiotic-dependent manner.},
journal = {Nature microbiology},
volume = {6},
number = {8},
issn = {2058-5276},
address = {London},
publisher = {Nature Publishing Group},
reportid = {DKFZ-2021-01538},
pages = {1043-1054},
year = {2021},
note = {#LA:F220# / 2021 Aug;6(8):1043-1054},
abstract = {Antimicrobial resistance poses a substantial threat to
human health. The gut microbiome is considered a reservoir
for potential spread of resistance genes from commensals to
pathogens, termed the gut resistome. The impact of
probiotics, commonly consumed by many in health or in
conjunction with the administration of antibiotics, on the
gut resistome is elusive. Reanalysis of gut metagenomes from
healthy antibiotics-naïve humans supplemented with an
11-probiotic-strain preparation, allowing direct assessment
of the gut resistome in situ along the gastrointestinal (GI)
tract, demonstrated that probiotics reduce the number of
antibiotic resistance genes exclusively in the gut of
colonization-permissive individuals. In mice and in a
separate cohort of humans, a course of antibiotics resulted
in expansion of the lower GI tract resistome, which was
mitigated by autologous faecal microbiome transplantation or
during spontaneous recovery. In contrast, probiotics further
exacerbated resistome expansion in the GI mucosa by
supporting the bloom of strains carrying vancomycin
resistance genes but not resistance genes encoded by the
probiotic strains. Importantly, the aforementioned effects
were not reflected in stool samples, highlighting the
importance of direct sampling to analyse the effect of
probiotics and antibiotics on the gut resistome. Analysing
antibiotic resistance gene content in additional published
clinical trials with probiotics further highlighted the
importance of person-specific metagenomics-based profiling
of the gut resistome using direct sampling. Collectively,
these findings suggest opposing person-specific and
antibiotic-dependent effects of probiotics on the resistome,
whose contribution to the spread of antimicrobial resistance
genes along the human GI tract merit further studies.},
cin = {F220},
ddc = {570},
cid = {I:(DE-He78)F220-20160331},
pnm = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
pid = {G:(DE-HGF)POF4-316},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34226711},
doi = {10.1038/s41564-021-00920-0},
url = {https://inrepo02.dkfz.de/record/169733},
}