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@ARTICLE{Reinle:169862,
author = {K. Reinle and A. Mogk and B. Bukau$^*$},
title = {{T}he diverse functions of small heat shock proteins in the
proteostasis network.},
journal = {Journal of molecular biology},
volume = {434},
number = {1},
issn = {0022-2836},
address = {Amsterdam [u.a.]},
publisher = {Elsevier},
reportid = {DKFZ-2021-01596},
pages = {167157},
year = {2022},
note = {#LA:A250# /2022 Jan 15;434(1):167157 / DKFZ-ZMBH Alliance},
abstract = {The protein quality control (PQC) system maintains protein
homeostasis by counteracting the accumulation of misfolded
protein conformers. Substrate degradation and refolding
activities executed by ATP-dependent proteases and
chaperones constitute major strategies of the proteostasis
network. Small heat shock proteins represent ATP-independent
chaperones that bind to misfolded proteins, preventing their
uncontrolled aggregation. sHsps share the conserved
α-crystallin domain (ACD) and gain functional specificity
through variable and largely disordered N- and C-terminal
extensions (NTE, CTE). They form large, polydisperse
oligomers through multiple, weak interactions between
NTE/CTEs and ACD dimers. Sequence variations of sHsps and
the large variability of sHsp oligomers enable sHsps to
fulfill diverse tasks in the PQC network. sHsp oligomers
represent inactive yet dynamic resting states that are
rapidly deoligomerized and activated upon stress conditions,
releasing substrate binding sites in NTEs and ACDs Bound
substrates are usually isolated in large sHsp/substrate
complexes. This sequestration activity of sHsps represents a
third strategy of the proteostasis network. Substrate
sequestration reduces the burden for other PQC components
during immediate and persistent stress conditions.
Sequestered substrates can be released and directed towards
refolding pathways by ATP-dependent Hsp70/Hsp100 chaperones
or sorted for degradation by autophagic pathways. sHsps can
also maintain the dynamic state of phase-separated stress
granules (SGs), which store mRNA and translation factors, by
reducing the accumulation of misfolded proteins inside SGs
and preventing unfolding of SG components. This ensures SG
disassembly and regain of translational capacity during
recovery periods.},
subtyp = {Review Article},
keywords = {chaperone (Other) / protein aggregation (Other) /
proteostasis (Other) / small heat shock protein (Other) /
stress granule (Other)},
cin = {A250},
ddc = {610},
cid = {I:(DE-He78)A250-20160331},
pnm = {311 - Zellbiologie und Tumorbiologie (POF4-311)},
pid = {G:(DE-HGF)POF4-311},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34271010},
doi = {10.1016/j.jmb.2021.167157},
url = {https://inrepo02.dkfz.de/record/169862},
}