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000169897 1001_ $$aSattler, Katherine$$b0
000169897 245__ $$aTRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes.
000169897 260__ $$a[London]$$bMacmillan Publishers Limited, part of Springer Nature$$c2021
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000169897 520__ $$aThe non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. The role of TRPV1 for myocardial inflammation has not been established yet. Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (hiPSC-CM) from 4 healthy donors were incubated with lipopolysaccharides (LPS, 6 h), TRPV1 agonist capsaicin (CAP, 20 min) or the antagonist capsazepine (CPZ, 20 min). TRPV1 expression was studied by PCR and western blotting. TRPV1 internalization was analyzed by immunofluorescence. Interleukin-6 (IL-6) secretion and phosphorylation of JNK, p38 and ERK were determined by ELISA. TRPV1-associated ion channel current was measured by patch clamp. TRPV1-mRNA and -protein were expressed in hiPSC-CM. TRPV1 was localized in the plasma membrane. LPS significantly increased secretion of IL-6 by 2.3-fold, which was prevented by pre-incubation with CPZ. LPS induced TRPV1 internalization. Phosphorylation levels of ERK, p38 or JNK were not altered by TRPV1 stimulation or inhibition. LPS and IL-6 significantly lowered TRPV1-mediated ion channel current. TRPV1 mediates the LPS-induced inflammation in cardiomyocytes, associated with changes of cellular electrophysiology. LPS-induced inflammation results in TRPV1 internalization. Further studies have to examine the underlying pathways and the clinical relevance of these findings.
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000169897 7001_ $$aEl-Battrawy, Ibrahim$$b1
000169897 7001_ $$00000-0001-9120-1382$$aCyganek, Lukas$$b2
000169897 7001_ $$aLang, Siegfried$$b3
000169897 7001_ $$aLan, Huan$$b4
000169897 7001_ $$0P:(DE-He78)128e4949aa4cb04fe109c52df0c67732$$aLi, Xin$$b5$$udkfz
000169897 7001_ $$aZhao, Zhihan$$b6
000169897 7001_ $$0P:(DE-He78)a229f7724466e7efadf4a1ace1ff8af3$$aUtikal, Jochen$$b7$$udkfz
000169897 7001_ $$aWieland, Thomas$$b8
000169897 7001_ $$aBorggrefe, Martin$$b9
000169897 7001_ $$aZhou, Xiaobo$$b10
000169897 7001_ $$aAkin, Ibrahim$$b11
000169897 773__ $$0PERI:(DE-600)2615211-3$$a10.1038/s41598-021-93958-3$$gVol. 11, no. 1, p. 14689$$n1$$p14689$$tScientific reports$$v11$$x2045-2322$$y2021
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