Home > Publications database > TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes. > print

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100 1 _ |a Sattler, Katherine
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245 _ _ |a TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes.
260 _ _ |a [London]
|c 2021
|b Macmillan Publishers Limited, part of Springer Nature
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520 _ _ |a The non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. The role of TRPV1 for myocardial inflammation has not been established yet. Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (hiPSC-CM) from 4 healthy donors were incubated with lipopolysaccharides (LPS, 6 h), TRPV1 agonist capsaicin (CAP, 20 min) or the antagonist capsazepine (CPZ, 20 min). TRPV1 expression was studied by PCR and western blotting. TRPV1 internalization was analyzed by immunofluorescence. Interleukin-6 (IL-6) secretion and phosphorylation of JNK, p38 and ERK were determined by ELISA. TRPV1-associated ion channel current was measured by patch clamp. TRPV1-mRNA and -protein were expressed in hiPSC-CM. TRPV1 was localized in the plasma membrane. LPS significantly increased secretion of IL-6 by 2.3-fold, which was prevented by pre-incubation with CPZ. LPS induced TRPV1 internalization. Phosphorylation levels of ERK, p38 or JNK were not altered by TRPV1 stimulation or inhibition. LPS and IL-6 significantly lowered TRPV1-mediated ion channel current. TRPV1 mediates the LPS-induced inflammation in cardiomyocytes, associated with changes of cellular electrophysiology. LPS-induced inflammation results in TRPV1 internalization. Further studies have to examine the underlying pathways and the clinical relevance of these findings.
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700 1 _ |a El-Battrawy, Ibrahim
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700 1 _ |a Cyganek, Lukas
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700 1 _ |a Lang, Siegfried
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700 1 _ |a Lan, Huan
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700 1 _ |a Zhao, Zhihan
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700 1 _ |a Utikal, Jochen
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700 1 _ |a Borggrefe, Martin
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700 1 _ |a Zhou, Xiaobo
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