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@ARTICLE{Jarahian:170400,
author = {M. Jarahian and K. Marstaller$^*$ and N. Banna$^*$ and R.
Ahani and M. H. Etemadzadeh and L. K. Boller and K.
Azadmanesh and A. Cid-Arregui$^*$ and A. Khezri and M. R.
Berger$^*$ and F. Momburg$^*$ and C. Watzl},
title = {{A}ctivating {N}atural {K}iller {C}ell {R}eceptors,
{S}electins, and {I}nhibitory {S}iglecs {R}ecognize
{E}bolavirus {G}lycoprotein.},
journal = {Journal of innate immunity},
volume = {14},
number = {2},
issn = {1662-8128},
address = {Sydney},
publisher = {Karger},
reportid = {DKFZ-2021-01907},
pages = {135-147},
year = {2022},
note = {#EA:G401# / 2022;14(2):135-147},
abstract = {Expression of the extensively glycosylated Ebolavirus
glycoprotein (EBOV-GP) induces physical alterations of
surface molecules and plays a crucial role in viral
pathogenicity. Here we investigate the interactions of
EBOV-GP with host surface molecules using purified EBOV-GP,
EBOV-GP-transfected cell lines, and EBOV-GP-pseudotyped
lentiviral particles. Subsequently, we wanted to examine
which receptors are involved in this recognition by binding
studies to cells transfected with the EBOV-GP as well as to
recombinant soluble EBOV-GP. As the viral components can
also bind to inhibitory receptors of immune cells (e.g.,
Siglecs, TIM-1), they can even suppress the activity of
immune effector cells. Our data show that natural killer
(NK) cell receptors NKp44 and NKp46, selectins (CD62E/P/L),
the host factors DC-SIGNR/DC-SIGN, and inhibitory Siglecs
function as receptors for EBOV-GP. Our results show also
moderate to strong avidity of homing receptors (P-, L-, and
E-selectin) and DC-SIGNR/DC-SIGN to purified EBOV-GP, to
cells transfected with EBOV-GP, as well as to the envelope
of a pseudotyped lentiviral vector carrying the EBOV-GP. The
concomitant activation and inhibition of the immune system
exemplifies the evolutionary antagonism between the immune
system and pathogens. Altogether these interactions with
activating and inhibitory receptors result in a reduced NK
cell-mediated lysis of EBOV-GP-expressing cells. Modulation
of these interactions may provide new strategies for
treating infections caused by this virus.},
keywords = {Ebolavirus glycoprotein (Other) / HPV (Other) / Natural
cytotoxicity receptors (Other) / Selectins (Other) / Siglecs
(Other)},
cin = {G401 / D122 / D121},
ddc = {610},
cid = {I:(DE-He78)G401-20160331 / I:(DE-He78)D122-20160331 /
I:(DE-He78)D121-20160331},
pnm = {314 - Immunologie und Krebs (POF4-314)},
pid = {G:(DE-HGF)POF4-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34425576},
doi = {10.1159/000517628},
url = {https://inrepo02.dkfz.de/record/170400},
}