%0 Journal Article
%A Marcu, Ana
%A Schlosser, Andreas
%A Keupp, Anne
%A Trautwein, Nico
%A Johann, Pascal
%A Wölfl, Matthias
%A Lager, Johanna
%A Monoranu, Camelia Maria
%A Walz, Juliane S
%A Henkel, Lisa M
%A Krauß, Jürgen
%A Ebinger, Martin
%A Schuhmann, Martin
%A Thomale, Ulrich Wilhelm
%A Pietsch, Torsten
%A Klinker, Erdwine
%A Schlegel, Paul G
%A Oyen, Florian
%A Reisner, Yair
%A Rammensee, Hans-Georg
%A Eyrich, Matthias
%T Natural and cryptic peptides dominate the immunopeptidome of atypical teratoid rhabdoid tumors.
%J Journal for ImmunoTherapy of Cancer
%V 9
%N 10
%@ 2051-1426
%C London
%I BioMed Central
%M DKFZ-2021-02090
%P e003404 
%D 2021
%X Atypical teratoid/rhabdoid tumors (AT/RT) are highly aggressive CNS tumors of infancy and early childhood. Hallmark is the surprisingly simple genome with inactivating mutations or deletions in the SMARCB1 gene as the oncogenic driver. Nevertheless, AT/RTs are infiltrated by immune cells and even clonally expanded T cells. However, it is unclear which epitopes T cells might recognize on AT/RT cells.Here, we report a comprehensive mass spectrometry (MS)-based analysis of naturally presented human leukocyte antigen (HLA) class I and class II ligands on 23 AT/RTs. MS data were validated by matching with a human proteome dataset and exclusion of peptides that are part of the human benignome. Cryptic peptide ligands were identified using Peptide-PRISM.Comparative HLA ligandome analysis of the HLA ligandome revealed 55 class I and 139 class II tumor-exclusive peptides. No peptide originated from the SMARCB1 region. In addition, 61 HLA class I tumor-exclusive peptide sequences derived from non-canonically translated proteins. Combination of peptides from natural and cryptic class I and class II origin gave optimal representation of tumor cell compartments. Substantial overlap existed with the cryptic immunopeptidome of glioblastomas, but no concordance was found with extracranial tumors. More than 80
%K antigens (Other)
%K epitope mapping (Other)
%K immunotherapy (Other)
%K neoplasm (Other)
%K pediatrics (Other)
%K vaccination (Other)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:34599019
%R 10.1136/jitc-2021-003404
%U https://inrepo02.dkfz.de/record/172542