000177187 001__ 177187
000177187 005__ 20240229133732.0
000177187 0247_ $$2doi$$a10.1016/j.canlet.2021.10.015
000177187 0247_ $$2pmid$$apmid:34688844
000177187 0247_ $$2ISSN$$a0304-3835
000177187 0247_ $$2ISSN$$a1872-7980
000177187 037__ $$aDKFZ-2021-02321
000177187 041__ $$aEnglish
000177187 082__ $$a570
000177187 1001_ $$0P:(DE-He78)80e100a16534f5fc67f7436ee67a47f9$$aRühle, Alexander$$b0$$eFirst author$$udkfz
000177187 245__ $$aHuman mesenchymal stromal cells maintain their stem cell traits after high-LET particle irradiation - Potential implications for particle radiotherapy and manned space missions.
000177187 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2021
000177187 3367_ $$2DRIVER$$aarticle
000177187 3367_ $$2DataCite$$aOutput Types/Journal article
000177187 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1635927763_31474
000177187 3367_ $$2BibTeX$$aARTICLE
000177187 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000177187 3367_ $$00$$2EndNote$$aJournal Article
000177187 500__ $$a#EA:E055#LA:E055#
000177187 520__ $$aThe influence of high-linear energy transfer (LET) particle radiation on the functionalities of mesenchymal stromal cells (MSCs) is largely unknown. Here, we analyzed the effects of proton (1H), helium (4He), carbon (12C) and oxygen (16O) ions on human bone marrow-MSCs. Cell cycle distribution and apoptosis induction were examined by flow cytometry, and DNA damage was quantified using γH2AX immunofluorescence and Western blots. Relative biological effectiveness values of MSCs amounted to 1.0-1.1 for 1H, 1.7-2.3 for 4He, 2.9-3.4 for 12C and 2.6-3.3 for 16O. Particle radiation did not alter the MSCs' characteristic surface marker pattern, and MSCs maintained their multi-lineage differentiation capabilities. Apoptosis rates ranged low for all radiation modalities. At 24 h after irradiation, particle radiation-induced ATM and CHK2 phosphorylation as well as γH2AX foci numbers returned to baseline levels. The resistance of human MSCs to high-LET irradiation suggests that MSCs remain functional after exposure to moderate doses of particle radiation as seen in normal tissues after particle radiotherapy or during manned space flights. In the future, in vivo models focusing on long-term consequences of particle irradiation on the bone marrow niche and MSCs are needed.
000177187 536__ $$0G:(DE-HGF)POF4-315$$a315 - Bildgebung und Radioonkologie (POF4-315)$$cPOF4-315$$fPOF IV$$x0
000177187 588__ $$aDataset connected to CrossRef, PubMed, , Journals: inrepo01.inet.dkfz-heidelberg.de
000177187 650_7 $$2Other$$aCarbon ions
000177187 650_7 $$2Other$$aHelium ions
000177187 650_7 $$2Other$$aHigh-LET radiation
000177187 650_7 $$2Other$$aMesenchymal stem cells
000177187 650_7 $$2Other$$aOxygen ions
000177187 650_7 $$2Other$$aParticle radiotherapy
000177187 650_7 $$2Other$$aProton radiotherapy
000177187 650_7 $$2Other$$aRadiotherapy
000177187 650_7 $$2Other$$aSpace radiation
000177187 7001_ $$0P:(DE-HGF)0$$aPing, Dai$$b1
000177187 7001_ $$0P:(DE-He78)c5a1a98649a7874de0def093eb136262$$aLopez Perez, Ramon$$b2$$udkfz
000177187 7001_ $$0P:(DE-HGF)0$$aStrack, Maren$$b3
000177187 7001_ $$aBrons, Stephan$$b4
000177187 7001_ $$aYijia, Qin$$b5
000177187 7001_ $$aDebus, Jürgen$$b6
000177187 7001_ $$aWuchter, Patrick$$b7
000177187 7001_ $$0P:(DE-HGF)0$$aGrosu, Anca-Ligia$$b8
000177187 7001_ $$0P:(DE-He78)3291aaac20f3d603d96744c1f0890028$$aHuber, Peter E$$b9$$udkfz
000177187 7001_ $$0P:(DE-He78)8d52e7ff1ccaac7dbf0232fdcb0168bd$$aNicolay, Nils$$b10$$eLast author$$udkfz
000177187 773__ $$0PERI:(DE-600)2004212-7$$a10.1016/j.canlet.2021.10.015$$gVol. 524, p. 172 - 181$$p172 - 181$$tCancer letters$$v524$$x0304-3835$$y2021
000177187 909CO $$ooai:inrepo02.dkfz.de:177187$$pVDB
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)80e100a16534f5fc67f7436ee67a47f9$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-HGF)0$$aDeutsches Krebsforschungszentrum$$b1$$kDKFZ
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)c5a1a98649a7874de0def093eb136262$$aDeutsches Krebsforschungszentrum$$b2$$kDKFZ
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-HGF)0$$aDeutsches Krebsforschungszentrum$$b3$$kDKFZ
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-HGF)0$$aDeutsches Krebsforschungszentrum$$b8$$kDKFZ
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)3291aaac20f3d603d96744c1f0890028$$aDeutsches Krebsforschungszentrum$$b9$$kDKFZ
000177187 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)8d52e7ff1ccaac7dbf0232fdcb0168bd$$aDeutsches Krebsforschungszentrum$$b10$$kDKFZ
000177187 9131_ $$0G:(DE-HGF)POF4-315$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vBildgebung und Radioonkologie$$x0
000177187 9141_ $$y2021
000177187 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz$$d2021-02-04$$wger
000177187 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bCANCER LETT : 2019$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bClarivate Analytics Master Journal List$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0160$$2StatID$$aDBCoverage$$bEssential Science Indicators$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)1190$$2StatID$$aDBCoverage$$bBiological Abstracts$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0113$$2StatID$$aWoS$$bScience Citation Index Expanded$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection$$d2021-02-04
000177187 915__ $$0StatID:(DE-HGF)9905$$2StatID$$aIF >= 5$$bCANCER LETT : 2019$$d2021-02-04
000177187 9201_ $$0I:(DE-He78)E055-20160331$$kE055$$lE055 KKE Translationale Radioonkologie$$x0
000177187 9201_ $$0I:(DE-He78)FR01-20160331$$kFR01$$lDKTK FR zentral$$x1
000177187 980__ $$ajournal
000177187 980__ $$aVDB
000177187 980__ $$aI:(DE-He78)E055-20160331
000177187 980__ $$aI:(DE-He78)FR01-20160331
000177187 980__ $$aUNRESTRICTED