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@ARTICLE{Holowatyj:177194,
      author       = {A. N. Holowatyj and B. Gigic and C. A. Warby and J. Ose and
                      T. Lin and P. Schrotz-King$^*$ and C. M. Ulrich and J. J.
                      Bernard},
      title        = {{T}he {U}se of {H}uman {S}erum {S}amples to {S}tudy
                      {M}alignant {T}ransformation: {A} {P}ilot {S}tudy.},
      journal      = {Cells},
      volume       = {10},
      number       = {10},
      issn         = {2073-4409},
      address      = {Basel},
      publisher    = {MDPI},
      reportid     = {DKFZ-2021-02328},
      pages        = {2670},
      year         = {2021},
      abstract     = {Obesity and excess adiposity account for approximately
                      $20\%$ of all cancer cases; however, biomarkers of risk
                      remain to be elucidated. While fibroblast growth factor-2
                      (FGF2) is emerging as an attractive candidate biomarker for
                      visceral adipose tissue mass, the role of circulating FGF2
                      in malignant transformation remains unknown. Moreover,
                      functional assays for biomarker discovery are limited. We
                      sought to determine if human serum could stimulate the 3D
                      growth of a non-tumorigenic cell line. This type of
                      anchorage-independent 3D growth in soft agar is a surrogate
                      marker for acquired tumorigenicity of cell lines. We found
                      that human serum from cancer-free men and women has the
                      potential to stimulate growth in soft agar of
                      non-tumorigenic epithelial JB6 P+ cells. We examined
                      circulating levels of FGF2 in humans in malignant
                      transformation in vitro in a pilot study of n = 33 men and
                      women. Serum FGF2 levels were not associated with colony
                      formation in epithelial cells (r = 0.05, p = 0.80); however,
                      a fibroblast growth factor receptor-1 (FGFR1) selective
                      inhibitor significantly blocked serum-stimulated
                      transformation, suggesting that FGF2 activation of FGFR1 may
                      be necessary, but not sufficient for the transforming
                      effects of human serum. This pilot study indicates that the
                      FGF2/FGFR1 axis plays a role in JB6 P+ malignant
                      transformation and describes an assay to determine critical
                      serum factors that have the potential to promote
                      tumorigenesis.},
      keywords     = {FGF2 (Other) / body fatness (Other) / body mass index
                      (Other) / obesity (Other) / overweight (Other)},
      cin          = {C120},
      ddc          = {570},
      cid          = {I:(DE-He78)C120-20160331},
      pnm          = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
      pid          = {G:(DE-HGF)POF4-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34685650},
      pmc          = {pmc:PMC8534413},
      doi          = {10.3390/cells10102670},
      url          = {https://inrepo02.dkfz.de/record/177194},
}