000177442 001__ 177442
000177442 005__ 20240229133740.0
000177442 037__ $$aDKFZ-2021-02534
000177442 1001_ $$0P:(DE-He78)02e29e2d540ff21d777f3a03ec9f5ade$$aJansen, Jeannette$$b0$$gfemale
000177442 245__ $$aOxygen Effects in (FLASH-) Radiotherapy on a Radiochemical and Genetic Level
000177442 260__ $$c2021
000177442 3367_ $$2DataCite$$aOutput Types/Dissertation
000177442 3367_ $$2ORCID$$aDISSERTATION
000177442 3367_ $$2BibTeX$$aPHDTHESIS
000177442 3367_ $$02$$2EndNote$$aThesis
000177442 3367_ $$0PUB:(DE-HGF)11$$2PUB:(DE-HGF)$$aDissertation / PhD Thesis$$bphd$$mphd$$s1637151973_22468
000177442 3367_ $$2DRIVER$$adoctoralThesis
000177442 500__ $$aCorresponding author J. Seco
000177442 502__ $$aDissertation, Universität Heidelberg, 2021$$bDissertation$$cUniversität Heidelberg$$gFakultät für Physik und Astronomie
000177442 520__ $$aIn radiotherapy, oxygen acts as strong radiosensitizer and alters cellular responseto radiation drastically. Especially, the e‚ect of the so-called FLASH radiotherapy,which applies high dose rates above 40 Gy/s to spare healthy tissue from radiationdamage, is inƒuenced by oxygen. At high O2levels the protective e‚ect of FLASH isdecreased in vivo. ‘e underlying mechanisms are not completely understood yet.In this thesis, oxygen e‚ects were investigated on (i) a radiochemical level by oxygen depletion measurements in water phantoms and together with cancer cells, (ii)on a genetic level developing a novel analysis method on gene expression pa‹ernsand (iii) on a mechanistic, radical scavenging level by modulating cellular defense.It was found, that a popular hypothesis for explaining the FLASH e‚ect, the oxygendepletion hypothesis, cannot be solely responsible for the observed altered cellularresponse a‰er FLASH through radiation induced hypoxia alone. However, radicalconcentrations are highly dependent on dose rate and beam pulse structure implyingpotential biological impact. Radical scavenging systems in cells were found to be altered using SOD-mimicking CuL/FeL compounds leading to a decrease of metastaticpotential. ‘e results strengthen the link between FLASH e‚ects and radical levels,inƒuenced by radical scavenging systems in cancer cells and oxygen conditions.
000177442 536__ $$0G:(DE-HGF)POF4-315$$a315 - Bildgebung und Radioonkologie (POF4-315)$$cPOF4-315$$fPOF IV$$x0
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000177442 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)02e29e2d540ff21d777f3a03ec9f5ade$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000177442 9131_ $$0G:(DE-HGF)POF4-315$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vBildgebung und Radioonkologie$$x0
000177442 9141_ $$y2021
000177442 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000177442 980__ $$aphd
000177442 980__ $$aVDB
000177442 980__ $$aI:(DE-He78)E041-20160331
000177442 980__ $$aUNRESTRICTED