TY  - THES
AU  - Jansen, Jeannette
TI  - Oxygen Effects in (FLASH-) Radiotherapy on a Radiochemical and Genetic Level
PB  - Universität Heidelberg
VL  - Dissertation
M1  - DKFZ-2021-02534
PY  - 2021
N1  - Corresponding author J. Seco
N1  - Dissertation, Universität Heidelberg, 2021
AB  - In radiotherapy, oxygen acts as strong radiosensitizer and alters cellular responseto radiation drastically. Especially, the e‚ect of the so-called FLASH radiotherapy,which applies high dose rates above 40 Gy/s to spare healthy tissue from radiationdamage, is inƒuenced by oxygen. At high O2levels the protective e‚ect of FLASH isdecreased in vivo. ‘e underlying mechanisms are not completely understood yet.In this thesis, oxygen e‚ects were investigated on (i) a radiochemical level by oxygen depletion measurements in water phantoms and together with cancer cells, (ii)on a genetic level developing a novel analysis method on gene expression pa‹ernsand (iii) on a mechanistic, radical scavenging level by modulating cellular defense.It was found, that a popular hypothesis for explaining the FLASH e‚ect, the oxygendepletion hypothesis, cannot be solely responsible for the observed altered cellularresponse a‰er FLASH through radiation induced hypoxia alone. However, radicalconcentrations are highly dependent on dose rate and beam pulse structure implyingpotential biological impact. Radical scavenging systems in cells were found to be altered using SOD-mimicking CuL/FeL compounds leading to a decrease of metastaticpotential. ‘e results strengthen the link between FLASH e‚ects and radical levels,inƒuenced by radical scavenging systems in cancer cells and oxygen conditions.
LB  - PUB:(DE-HGF)11
UR  - https://inrepo02.dkfz.de/record/177442
ER  -