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@PHDTHESIS{Jansen:177442,
author = {J. Jansen$^*$},
title = {{O}xygen {E}ffects in ({FLASH}-) {R}adiotherapy on a
{R}adiochemical and {G}enetic {L}evel},
school = {Universität Heidelberg},
type = {Dissertation},
reportid = {DKFZ-2021-02534},
year = {2021},
note = {Corresponding author J. Seco; Dissertation, Universität
Heidelberg, 2021},
abstract = {In radiotherapy, oxygen acts as strong radiosensitizer and
alters cellular responseto radiation drastically.
Especially, the eect of the so-called FLASH
radiotherapy,which applies high dose rates above 40 Gy/s to
spare healthy tissue from radiationdamage, is inuenced by
oxygen. At high O2levels the protective eect of FLASH
isdecreased in vivo. e underlying mechanisms are not
completely understood yet.In this thesis, oxygen eects
were investigated on (i) a radiochemical level by oxygen
depletion measurements in water phantoms and together with
cancer cells, (ii)on a genetic level developing a novel
analysis method on gene expression paernsand (iii) on a
mechanistic, radical scavenging level by modulating cellular
defense.It was found, that a popular hypothesis for
explaining the FLASH eect, the oxygendepletion hypothesis,
cannot be solely responsible for the observed altered
cellularresponse aer FLASH through radiation induced
hypoxia alone. However, radicalconcentrations are highly
dependent on dose rate and beam pulse structure
implyingpotential biological impact. Radical scavenging
systems in cells were found to be altered using
SOD-mimicking CuL/FeL compounds leading to a decrease of
metastaticpotential. e results strengthen the link between
FLASH eects and radical levels,inuenced by radical
scavenging systems in cancer cells and oxygen conditions.},
cin = {E041},
cid = {I:(DE-He78)E041-20160331},
pnm = {315 - Bildgebung und Radioonkologie (POF4-315)},
pid = {G:(DE-HGF)POF4-315},
typ = {PUB:(DE-HGF)11},
url = {https://inrepo02.dkfz.de/record/177442},
}