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@PHDTHESIS{Hanley:177443,
author = {R. Hanley$^*$},
title = {{S}tudying radiation generated {DNA} damage and how it is
affected by oxygen and cellular metabolism},
school = {Universität Heidelberg},
type = {Dissertation},
reportid = {DKFZ-2021-02535},
year = {2021},
note = {Corresponding author J. Seco; Dissertation, Universität
Heidelberg, 2021},
abstract = {Cancer is one of the leading causes of death in the world
and is routinely treated with radiotherapy. Radiation causes
cellular damage by interacting either directly with DNA or
indirectly via the formation of reactive oxygen species
(ROS) resulting in double strand breaks (DSBs) and/or
genomic instability. However, the efficacy of the radiation
depends largely on the tumor microenvironment. Tumors tend
to form hypoxic (low oxygen) regions due to their highly
proliferative characteristics but are biologically
programmed to adapt to these low oxygen situations via
metabolic alterations to maintain their homeostasis. This
can be problematic, however, as it can have a strong
negative influence on the biological effects of ionizing
radiation by increasing cellular radio-resistance and
thereby reducing the therapy outcome (1,2).There are
therefore two aims to this dissertation: 1. Analyse the
indirect DNA damage of radiation on different non-small lung
cancer cell lines (NSCLC) and correlate with nuclear and
cytosolic radiation-induced ROS.2. Study how these adaptive
responses to tumor hypoxia contribute to radio-resistance
for a variety of different cancer cell lines and thus
provide potential metabolic predictors to therapy outcome.},
cin = {E041},
cid = {I:(DE-He78)E041-20160331},
pnm = {315 - Bildgebung und Radioonkologie (POF4-315)},
pid = {G:(DE-HGF)POF4-315},
typ = {PUB:(DE-HGF)11},
url = {https://inrepo02.dkfz.de/record/177443},
}