000177449 001__ 177449
000177449 005__ 20240229133740.0
000177449 037__ $$aDKFZ-2021-02541
000177449 1001_ $$0P:(DE-He78)e4bc103f9e4a73fca91dbd876ed703d4$$aBrieger, Emily$$b0
000177449 245__ $$aCharacterisation and comparison ofsmall extracellular vesicles after irradiationwith X-rays versus Carbon ions
000177449 260__ $$c2021
000177449 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000177449 3367_ $$02$$2EndNote$$aThesis
000177449 3367_ $$2BibTeX$$aMASTERSTHESIS
000177449 3367_ $$2DRIVER$$amasterThesis
000177449 3367_ $$0PUB:(DE-HGF)19$$2PUB:(DE-HGF)$$aMaster Thesis$$bmaster$$mmaster$$s1637151697_22468
000177449 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000177449 500__ $$aCorresponding author J. Seco
000177449 502__ $$aMasterarbeit, Universität Heidelberg, 2021$$bMasterarbeit$$cUniversität Heidelberg$$gFakultät für Physik und Astronomie
000177449 520__ $$aPancreatic cancer is one of the most aggressive cancer types with poor prognosisdue to treatment resistance. Small extracellular vesicles (sEVs) are secreted byalmost all cell types and have been shown to be involved in several steps of cancerprogression and treatment resistance. To date, only a limited number of studiesinvestigated the effect of irradiation, especially heavy charged particle irradiation,on sEV secretion and composition. In this thesis, sEVs were isolated from twopancreatic cancer cell lines (Panc01, KPC) after irradiation with X-rays or carbonions. It was observed that both irradiation modalities stimulate the biogenesis andrelease of sEVs without influencing their size. Moreover, a connection betweenthe autophagy pathway and sEV biogenesis was observed. Investigation of theprotein content pointed at a change in sEV composition after irradiation, sincethe expression of several sEV markers and proteins increased after irradiation.In addition, double strand DNA (dsDNA) associated with sEVs was extensivelyexamined. It was found that the amount of dsDNA increased with increasingdose until 6Gy (X-rays) or 3Gy (Carbon ions), possibly indicating an activationof a degradation mechanisms at higher doses. Following experiments showed thatthe dsDNA was predominately localised on the surface of sEVs. Shedding lighton the effects of radiotherapy (X-rays vs carbon ions) on sEV composition is afirst step towards unlocking the full potential of sEVs in radiotherapy-associatedprognosis for pancreatic cancer, and unravelling new potential target for combinedtherapies.
000177449 536__ $$0G:(DE-HGF)POF4-315$$a315 - Bildgebung und Radioonkologie (POF4-315)$$cPOF4-315$$fPOF IV$$x0
000177449 909CO $$ooai:inrepo02.dkfz.de:177449$$pVDB
000177449 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)e4bc103f9e4a73fca91dbd876ed703d4$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000177449 9131_ $$0G:(DE-HGF)POF4-315$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vBildgebung und Radioonkologie$$x0
000177449 9141_ $$y2021
000177449 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000177449 980__ $$amaster
000177449 980__ $$aVDB
000177449 980__ $$aI:(DE-He78)E041-20160331
000177449 980__ $$aUNRESTRICTED