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@MASTERSTHESIS{Brieger:177449,
      author       = {E. Brieger$^*$},
      title        = {{C}haracterisation and comparison ofsmall extracellular
                      vesicles after irradiationwith {X}-rays versus {C}arbon
                      ions},
      school       = {Universität Heidelberg},
      type         = {Masterarbeit},
      reportid     = {DKFZ-2021-02541},
      year         = {2021},
      note         = {Corresponding author J. Seco; Masterarbeit, Universität
                      Heidelberg, 2021},
      abstract     = {Pancreatic cancer is one of the most aggressive cancer
                      types with poor prognosisdue to treatment resistance. Small
                      extracellular vesicles (sEVs) are secreted byalmost all cell
                      types and have been shown to be involved in several steps of
                      cancerprogression and treatment resistance. To date, only a
                      limited number of studiesinvestigated the effect of
                      irradiation, especially heavy charged particle
                      irradiation,on sEV secretion and composition. In this
                      thesis, sEVs were isolated from twopancreatic cancer cell
                      lines (Panc01, KPC) after irradiation with X-rays or
                      carbonions. It was observed that both irradiation modalities
                      stimulate the biogenesis andrelease of sEVs without
                      influencing their size. Moreover, a connection betweenthe
                      autophagy pathway and sEV biogenesis was observed.
                      Investigation of theprotein content pointed at a change in
                      sEV composition after irradiation, sincethe expression of
                      several sEV markers and proteins increased after
                      irradiation.In addition, double strand DNA (dsDNA)
                      associated with sEVs was extensivelyexamined. It was found
                      that the amount of dsDNA increased with increasingdose until
                      6Gy (X-rays) or 3Gy (Carbon ions), possibly indicating an
                      activationof a degradation mechanisms at higher doses.
                      Following experiments showed thatthe dsDNA was predominately
                      localised on the surface of sEVs. Shedding lighton the
                      effects of radiotherapy (X-rays vs carbon ions) on sEV
                      composition is afirst step towards unlocking the full
                      potential of sEVs in radiotherapy-associatedprognosis for
                      pancreatic cancer, and unravelling new potential target for
                      combinedtherapies.},
      cin          = {E041},
      cid          = {I:(DE-He78)E041-20160331},
      pnm          = {315 - Bildgebung und Radioonkologie (POF4-315)},
      pid          = {G:(DE-HGF)POF4-315},
      typ          = {PUB:(DE-HGF)19},
      url          = {https://inrepo02.dkfz.de/record/177449},
}