000177451 001__ 177451
000177451 005__ 20240229133740.0
000177451 037__ $$aDKFZ-2021-02543
000177451 1001_ $$0P:(DE-He78)76034c0252d4d77ae655719fc0fb3a44$$aBehr, Carola$$b0
000177451 245__ $$aDetectability of radiation induced H2O2 with lowconcentrated fluorophores NucPE1 and PY1-ME
000177451 260__ $$c2021
000177451 3367_ $$2DRIVER$$abachelorThesis
000177451 3367_ $$02$$2EndNote$$aThesis
000177451 3367_ $$2DataCite$$aOutput Types/Supervised Student Publication
000177451 3367_ $$0PUB:(DE-HGF)2$$2PUB:(DE-HGF)$$aBachelor Thesis$$bbachelor$$mbachelor$$s1637151582_19386
000177451 3367_ $$2BibTeX$$aMASTERSTHESIS
000177451 3367_ $$2ORCID$$aSUPERVISED_STUDENT_PUBLICATION
000177451 500__ $$aCorresponding author J. Seco
000177451 502__ $$aBachelorarbeit, Universität Heidelberg, 2021$$bBachelorarbeit$$cUniversität Heidelberg$$gFakultät für Physik und Astronomie
000177451 520__ $$aA new method to quantify the amount of DNA damage due to irradiation is currentlydeveloped for applications in radiation therapy research. It quantifies the amount ofDNA damage by measuring the amount of produced hydrogen peroxide H2O2. This willbe measured inside cells with the fluorophores Nuclear Peroxy Emerald 1(NucPE1) andPeroxy Yellow-1 Methyl Ester (PY1-ME).In this thesis the number of molecules of each dye that localise inside a cell of the cell lineH460 was analysed. This resulted in an amount of (1.4±0.3)108 moleculescell for NucPE1 and(1 ± 3)109 moleculescell for PY1-ME.In addition a function to calibrate a number n of H460 cells to a concentration c was foundto be c = f ∗ n + bg. 100 µL of a solution with concentration c of the fluorophore producea signal of the same magnitude as n cells. The parameters of this function were found tobe fNucP E1 = (2.4 ± 0.5)10−6 µMcell and bgNucP E1 = (0 ± 1.8) µM for NucPE1. For PY1-MEthe parameters resulted in fP Y 1−ME = (2 ± 5)10−5 µMcell and bgP Y 1−ME = (3 ± 82) µM.Moreover, it was possible to detect the increase of fluorescence signal with increasing dosefor a number of molecules like there are in 5000 H460 cells. This was realised with a platereader and a photon counting system.The results confirm the possibilty to developing a chemical dosimeter for in cell measurements based on one of these fluorophores.
000177451 536__ $$0G:(DE-HGF)POF4-315$$a315 - Bildgebung und Radioonkologie (POF4-315)$$cPOF4-315$$fPOF IV$$x0
000177451 909CO $$ooai:inrepo02.dkfz.de:177451$$pVDB
000177451 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)76034c0252d4d77ae655719fc0fb3a44$$aDeutsches Krebsforschungszentrum$$b0$$kDKFZ
000177451 9131_ $$0G:(DE-HGF)POF4-315$$1G:(DE-HGF)POF4-310$$2G:(DE-HGF)POF4-300$$3G:(DE-HGF)POF4$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vBildgebung und Radioonkologie$$x0
000177451 9141_ $$y2021
000177451 9201_ $$0I:(DE-He78)E041-20160331$$kE041$$lE041 Medizinische Physik in der Radioonkologie$$x0
000177451 980__ $$abachelor
000177451 980__ $$aVDB
000177451 980__ $$aI:(DE-He78)E041-20160331
000177451 980__ $$aUNRESTRICTED