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@MASTERSTHESIS{Behr:177451,
      author       = {C. Behr$^*$},
      title        = {{D}etectability of radiation induced {H}2{O}2 with
                      lowconcentrated fluorophores {N}uc{PE}1 and {PY}1-{ME}},
      school       = {Universität Heidelberg},
      type         = {Bachelorarbeit},
      reportid     = {DKFZ-2021-02543},
      year         = {2021},
      note         = {Corresponding author J. Seco; Bachelorarbeit, Universität
                      Heidelberg, 2021},
      abstract     = {A new method to quantify the amount of DNA damage due to
                      irradiation is currentlydeveloped for applications in
                      radiation therapy research. It quantifies the amount ofDNA
                      damage by measuring the amount of produced hydrogen peroxide
                      H2O2. This willbe measured inside cells with the
                      fluorophores Nuclear Peroxy Emerald 1(NucPE1) andPeroxy
                      Yellow-1 Methyl Ester (PY1-ME).In this thesis the number of
                      molecules of each dye that localise inside a cell of the
                      cell lineH460 was analysed. This resulted in an amount of
                      (1.4±0.3)108 moleculescell for NucPE1 and(1 ± 3)109
                      moleculescell for PY1-ME.In addition a function to calibrate
                      a number n of H460 cells to a concentration c was foundto be
                      c = f ∗ n + bg. 100 µL of a solution with concentration c
                      of the fluorophore producea signal of the same magnitude as
                      n cells. The parameters of this function were found tobe
                      fNucP E1 = (2.4 ± 0.5)10−6 µMcell and bgNucP E1 = (0 ±
                      1.8) µM for NucPE1. For PY1-MEthe parameters resulted in fP
                      Y 1−ME = (2 ± 5)10−5 µMcell and bgP Y 1−ME = (3 ±
                      82) µM.Moreover, it was possible to detect the increase of
                      fluorescence signal with increasing dosefor a number of
                      molecules like there are in 5000 H460 cells. This was
                      realised with a platereader and a photon counting system.The
                      results confirm the possibilty to developing a chemical
                      dosimeter for in cell measurements based on one of these
                      fluorophores.},
      cin          = {E041},
      cid          = {I:(DE-He78)E041-20160331},
      pnm          = {315 - Bildgebung und Radioonkologie (POF4-315)},
      pid          = {G:(DE-HGF)POF4-315},
      typ          = {PUB:(DE-HGF)2},
      url          = {https://inrepo02.dkfz.de/record/177451},
}