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| 001 | 177453 | ||
| 005 | 20240229133740.0 | ||
| 037 | _ | _ | |a DKFZ-2021-02545 |
| 100 | 1 | _ | |a Da Silva Azevedo, Jessica |0 P:(DE-He78)034c68cc0a19c04c32bc654c40ceceb9 |b 0 |
| 245 | _ | _ | |a Interconnection between cancer derived small extracellular vesicles and lipid droplets |
| 260 | _ | _ | |c 2021 |
| 336 | 7 | _ | |a bachelorThesis |2 DRIVER |
| 336 | 7 | _ | |a Thesis |0 2 |2 EndNote |
| 336 | 7 | _ | |a Output Types/Supervised Student Publication |2 DataCite |
| 336 | 7 | _ | |a Bachelor Thesis |b bachelor |m bachelor |0 PUB:(DE-HGF)2 |s 1637151491_7660 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a MASTERSTHESIS |2 BibTeX |
| 336 | 7 | _ | |a SUPERVISED_STUDENT_PUBLICATION |2 ORCID |
| 500 | _ | _ | |a Corresponding author J. Seco |
| 502 | _ | _ | |a Bachelorarbeit, University of Applied Sciences Mannheim, 2021 |c University of Applied Sciences Mannheim |b Bachelorarbeit |g Department of Biotechnology |
| 520 | _ | _ | |a Small extracellular vesicles (sEVs) are nanovesicles enclosed by a phospholipid bilayer membrane and are produced by both healthy and malignant cells. sEVs are responsible for cell-to-cell communication and have been described as performing a variety of functions that alter the physiological or pathological state of the recipient cells. They can enhance tumor development and progression by promoting metastasis and angiogenesis via regulation of the tumoral microenvironment [1]. All these factors have led to the growing interest in discovering potential future therapeutic sEV-based approaches, such as drug delivery vehicles. Increasing evidence indicates that lipid metabolism influences sEV biological activities such as biosyn-thesis and interactions with recipient cells. This is likely related to the fact that lipids that are stored and released from lipid droplets (LDs) are key components of the plasma membrane, which can reprogram their fluidity contributing to an optimal environment for the exchange of genetic material between cells. The aim of this project was to investigate the correlation be-tween the release of tumor-derived sEVs and the biogenesis of lipid droplets. In order to study this connection, several types of experiments were used in this bachelor thesis. Previous studies have shown evidence that tumor acidity and sEV biogenesis represent common can-cer features [2] along with the confirmation that X-ray activates sEV biogenesis and secretion [3]. Therefore, this project was focused to investigate how the stimulation by these conditions affected the biogenesis of sEVs and lipid droplets in cancer cell lines isolated from the breast (MCF7) and lung (H460) tumors. To this purpose, the two cell lines were cultured in either a neutral pH (7.4) or an acidic pH (6.5) typical of tumor environment [4]. Additionally, cell lines were irradiated with 6 Gy for the radiation experiment. The number of released sEVs was measured by fluorometric protein quantification and nanoparticle tracking analysis (NTA). The expression of sEV protein markers was examined by Western blotting. Moreover, sEVs were characterized by electron microscopy. The produced LD number per cell was obtained through fluorescent confocal microscopy. Furthermore, two tumor-derived colon cell lines, HT29 and LoVo, were directly compared for the biogenesis of sEVs and lipid droplets. Previ-ous studies showed that LoVo has small amounts of LDs within cells in comparison to HT29 [5]. For this study, Lovo was perceived as the control and compared to HT29 which has a natural higher LD accumulation. The experimental setup was essentially the same with the exception that cells were not exposed to treatment. The results showed that pH 6.5 and 6 Gy induced a remarkable increase in sEVs and LD production, and the control groups had the opposite outcome for all cancer cell lines. The cell lines LoVo and HT29, which were not in-duced by artificial conditioning, showed an experimental model which reflects natural settings. The outcome confirmed the hypothesis that there is an interconnection in the biogenesis pathway between the two cell organelles, but further in-depth research is needed to uncover the mechanistic processes underlying this potential connection. |
| 536 | _ | _ | |a 315 - Bildgebung und Radioonkologie (POF4-315) |0 G:(DE-HGF)POF4-315 |c POF4-315 |f POF IV |x 0 |
| 909 | C | O | |o oai:inrepo02.dkfz.de:177453 |p VDB |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 0 |6 P:(DE-He78)034c68cc0a19c04c32bc654c40ceceb9 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Krebsforschung |1 G:(DE-HGF)POF4-310 |0 G:(DE-HGF)POF4-315 |3 G:(DE-HGF)POF4 |2 G:(DE-HGF)POF4-300 |4 G:(DE-HGF)POF |v Bildgebung und Radioonkologie |x 0 |
| 914 | 1 | _ | |y 2021 |
| 920 | 1 | _ | |0 I:(DE-He78)E041-20160331 |k E041 |l E041 Medizinische Physik in der Radioonkologie |x 0 |
| 980 | _ | _ | |a bachelor |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-He78)E041-20160331 |
| 980 | _ | _ | |a UNRESTRICTED |
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