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@INBOOK{Mansour:177487,
      author       = {P. Mansour$^*$ and I. Quiros Fernandez$^*$ and E. Fakhr$^*$
                      and A. Cid-Arregui$^*$},
      title        = {{G}eneration of {CAR}-{T} cells using lentiviral vectors},
      address      = {United States},
      publisher    = {Academic Press Inc.},
      reportid     = {DKFZ-2021-02574},
      pages        = {-},
      year         = {2021},
      comment      = {Methods in Cell Biology},
      booktitle     = {Methods in Cell Biology},
      abstract     = {Cancer immunotherapy is nowadays largely focused on the
                      development of therapeutic antibodies and chimeric antigen
                      receptors (CARs). Two CARs targeting CD19 have been approved
                      recently for the treatment of some hematological
                      malignancies. This demonstrates the capability of engineered
                      CAR T cells in generating effective tumor responses.
                      Furthermore, several hundred ongoing clinical trials are
                      exploring the feasibility of CAR-based approaches to target
                      tumor-associated antigens in solid tumors. However, there
                      still remain significant challenges and limitations in the
                      design and production of CAR-modified T cells that need to
                      be addressed, such as more effective transduction methods,
                      expression and exhaustion issues, reliable in vitro and in
                      vivo characterization methods, etc. Here we describe current
                      techniques for generating CAR T cells using lentiviral
                      vectors as well as detailed protocols for their functional
                      $characterization.https://reader.elsevier.com/reader/sd/pii/S0091679X21000844?token=19B8578AB0A842C9E0A423517A3E8821C298512484E57C501C77B440E558856281E370B178DDAB9F208EBEE22E875043\&originRegion=eu-west-1\&originCreation=20211118072303$},
      cin          = {D122},
      cid          = {I:(DE-He78)D122-20160331},
      pnm          = {314 - Immunologie und Krebs (POF4-314)},
      pid          = {G:(DE-HGF)POF4-314},
      typ          = {PUB:(DE-HGF)7},
      url          = {https://inrepo02.dkfz.de/record/177487},
}