TY  - JOUR
AU  - Wohlfarth, Daniel
AU  - Frehtman, Veronika
AU  - Müller, Marcus
AU  - Vogel, Martin
AU  - Phan, Linh Minh Phuc
AU  - Brunecker, Adrian
AU  - Leuchs, Barbara
TI  - Upstream process optimization and micro- and macrocarrier screening for large-scale production of the oncolytic H-1 protoparvovirus.
JO  - Applied microbiology and biotechnology
VL  - 105
IS  - 24
SN  - 1432-0614
CY  - New York
PB  - Springer
M1  - DKFZ-2021-02580
SP  - 9113-9124
PY  - 2021
N1  - #EA:F010#LA:F010# / 2021 Dec;105(24):9113-9124
AB  - The oncolytic virus H-1PV is a promising candidate for various cancer treatments. Therefore, production process needs to be optimized and scaled up for future market release. Currently, the virus is produced with minimum essential medium in 10-layer CellSTACK® chambers with limited scalability, requiring a minimum seeding density of 7.9E3 cells/cm2. Production also requires a 5
KW  - Bead-to-bead transfer (Other)
KW  - Macrocarrier (Other)
KW  - Microcarrier (Other)
KW  - Protoparvovirus H-1PV production (Other)
KW  - Scale-up (Other)
KW  - Serum-free (Other)
LB  - PUB:(DE-HGF)16
C6  - pmid:34782922
DO  - DOI:10.1007/s00253-021-11642-y
UR  - https://inrepo02.dkfz.de/record/177493
ER  -