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@ARTICLE{Guo:177877,
author = {C. Guo and D. Reuss and J. D. Coey and S. Sukumar and B.
Lang and J. McLauchlan and S. Boulant$^*$ and M. L. Stanifer
and C. G. G. Bamford},
title = {{C}onserved {I}nduction of {D}istinct {A}ntiviral
{S}ignalling {K}inetics by {P}rimate {I}nterferon {L}ambda 4
{P}roteins.},
journal = {Frontiers in immunology},
volume = {12},
issn = {1664-3224},
address = {Lausanne},
publisher = {Frontiers Media},
reportid = {DKFZ-2021-02902},
pages = {772588},
year = {2021},
abstract = {Interferon lambdas (IFNλ) (also known as type III IFNs)
are critical cytokines that combat infection predominantly
at barrier tissues, such as the lung, liver, and
gastrointestinal tract. Humans have four IFNλs (1-4), where
IFNλ1-3 show $~80\%-95\%$ homology, and IFNλ4 is the most
divergent displaying only $~30\%$ sequence identity.
Variants in IFNλ4 in humans are associated with the outcome
of infection, such as with hepatitis C virus. However, how
IFNλ4 variants impact cytokine signalling in other tissues
and how well this is conserved is largely unknown. In this
study, we address whether differences in antiviral
signalling exist between IFNλ4 variants in human hepatocyte
and intestinal cells, comparing them to IFNλ3. We
demonstrate that compared to IFNλ3, wild-type human IFNλ4
induces a signalling response with distinct magnitudes and
kinetics, which is modified by naturally occurring variants
P70S and K154E in both cell types. IFNλ4's distinct
antiviral response was more rapid yet transient compared to
IFNλ1 and 3. Additionally, divergent antiviral kinetics
were also observed using non-human primate IFNλs and cell
lines. Furthermore, an IFNλ4-like receptor-interacting
interface failed to alter IFNλ1's kinetics. Together, our
data provide further evidence that major functional
differences exist within the IFNλ gene family. These
results highlight the possible tissue specialisation of
IFNλs and encourage further investigation of the divergent,
non-redundant activities of IFNλ4 and other IFNλs.},
keywords = {IFNL4 (Other) / antiviral (Other) / interferon (Other) /
kinetics (Other) / lambda (Other) / signalling (Other)},
cin = {F140},
ddc = {610},
cid = {I:(DE-He78)F140-20160331},
pnm = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
pid = {G:(DE-HGF)POF4-316},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:34868037},
pmc = {pmc:PMC8636442},
doi = {10.3389/fimmu.2021.772588},
url = {https://inrepo02.dkfz.de/record/177877},
}