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@ARTICLE{RoigMerino:178288,
      author       = {A. Roig-Merino$^*$ and M. Urban$^*$ and M. Bozza$^*$ and J.
                      D. Peterson$^*$ and L. Bullen and M. Büchler-Schäff$^*$
                      and S. Stäble$^*$ and F. van der Hoeven$^*$ and K.
                      Müller-Decker$^*$ and T. R. McKay and M. D. Milsom$^*$ and
                      R. Harbottle$^*$},
      title        = {{A}n episomal {DNA} vector platform for the persistent
                      genetic modification of pluripotent stem cells and their
                      differentiated progeny.},
      journal      = {Stem cell reports},
      volume       = {17},
      number       = {1},
      issn         = {2213-6711},
      address      = {[New York, NY]},
      publisher    = {Elsevier},
      reportid     = {DKFZ-2021-03235},
      pages        = {143-158},
      year         = {2022},
      note         = {#EA:F160#LA:F160# /2022 Jan 11;17(1):143-158},
      abstract     = {The genetic modification of stem cells (SCs) is typically
                      achieved using integrating vectors, whose potential
                      integrative genotoxicity and propensity for epigenetic
                      silencing during differentiation limit their application.
                      The genetic modification of cells should provide sustainable
                      levels of transgene expression, without compromising the
                      viability of a cell or its progeny. We developed nonviral,
                      nonintegrating, and autonomously replicating minimally sized
                      DNA nanovectors to persistently genetically modify SCs and
                      their differentiated progeny without causing any molecular
                      or genetic damage. These DNA vectors are capable of
                      efficiently modifying murine and human pluripotent SCs with
                      minimal impact and without differentiation-mediated
                      transgene silencing or vector loss. We demonstrate that
                      these vectors remain episomal and provide robust and
                      sustained transgene expression during self-renewal and
                      targeted differentiation of SCs both in vitro and in vivo
                      through embryogenesis and differentiation into adult
                      tissues, without damaging their phenotypic characteristics.},
      keywords     = {DNA vector (Other) / SMAR (Other) / differentiation (Other)
                      / embryonic stem cell (Other) / episome (Other) / induced
                      pluripotent stem cell (Other) / nonintegrative (Other) /
                      reprogramming (Other) / self-renewal (Other) / transgenesis
                      (Other)},
      cin          = {F160 / A012 / B340 / W450 / W420},
      ddc          = {610},
      cid          = {I:(DE-He78)F160-20160331 / I:(DE-He78)A012-20160331 /
                      I:(DE-He78)B340-20160331 / I:(DE-He78)W450-20160331 /
                      I:(DE-He78)W420-20160331},
      pnm          = {316 - Infektionen, Entzündung und Krebs (POF4-316)},
      pid          = {G:(DE-HGF)POF4-316},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34942088},
      doi          = {10.1016/j.stemcr.2021.11.011},
      url          = {https://inrepo02.dkfz.de/record/178288},
}