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@ARTICLE{Bojcevski:178296,
      author       = {J. Bojcevski$^*$ and C. Wang$^*$ and H. Liu$^*$ and A.
                      Abdollahi$^*$ and I. Dokic$^*$},
      title        = {{A}ssessment of {N}ormal {T}issue {R}adiosensitivity by
                      {E}valuating {DNA} {D}amage and {R}epair {K}inetics in
                      {H}uman {B}rain {O}rganoids.},
      journal      = {International journal of molecular sciences},
      volume       = {22},
      number       = {24},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {DKFZ-2021-03243},
      pages        = {13195},
      year         = {2021},
      note         = {#EA:E210#LA:E210#},
      abstract     = {DNA-double strand break (DSB), detected by immunostaining
                      of key proteins orchestrating repair, like γH2AX and 53BP1,
                      is well established as a surrogate for tissue
                      radiosensitivity. We hypothesized that the generation of
                      normal brain 3D organoids ('mini-brains') from human induced
                      pluripotent stem cells (hiPSC) combined with detection of
                      DNA damage repair (DDR) may hold the promise towards
                      developing personalized models for the determination of
                      normal tissue radiosensitivity. In this study, cerebral
                      organoids, an in vitro model that stands in its complexity
                      between 2D cellular system and an organ, have been used. To
                      quantify radiation-induced response, immunofluorescent
                      staining with γH2AX and 53BP1 were applied at early (30
                      min, initial damage), and late time points (18 and 72 h,
                      residual damage), following clinical standard 2 Gy
                      irradiation. Based on our findings, assessment of DDR
                      kinetics as a surrogate for radiosensitivity in hiPSC
                      derived cerebral organoids is feasible. Further development
                      of mini-brains recapitulating mature adult neuronal tissue
                      and implementation of additional signaling and toxicity
                      surrogates may pave the way towards development of
                      next-generation personalized assessment of radiosensitivity
                      in healthy neuronal tissue.},
      keywords     = {DNA damage repair (Other) / brain organoids (Other) /
                      mini-brains (Other) / neurotoxicity (Other) / radiation
                      (Other) / radiosensitivity (Other)},
      cin          = {E210 / HD01 / A240},
      ddc          = {540},
      cid          = {I:(DE-He78)E210-20160331 / I:(DE-He78)HD01-20160331 /
                      I:(DE-He78)A240-20160331},
      pnm          = {315 - Bildgebung und Radioonkologie (POF4-315)},
      pid          = {G:(DE-HGF)POF4-315},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:34947991},
      doi          = {10.3390/ijms222413195},
      url          = {https://inrepo02.dkfz.de/record/178296},
}