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@ARTICLE{Lam:178524,
author = {S. Y. Lam and M. C. Mommersteeg and B. Yu and L. Broer and
M. C. W. Spaander and F. Frost and S. Weiss and H. Völzke
and M. M. Lerch and B. Schöttker$^*$ and Y. Zhang$^*$ and
H. Stocker$^*$ and H. Brenner$^*$ and D. Levy and S.-J.
Hwang and A. C. Wood and S. S. Rich and J. I. Rotter and K.
D. Taylor and R. P. Tracy and E. K. Kabagambe and M. Leja
and J. Klovins and R. Peculis and D. Rudzite and L.
Nikitina-Zake and G. Skenders and V. Rovite and A.
Uitterlinden and E. J. Kuipers and G. M. Fuhler and G.
Homuth and M. P. Peppelenbosch},
title = {{T}oll-like receptor 1 locus re-examined in a genome-wide
association study update on anti-{H}elicobacter pylori
{I}g{G} titers.},
journal = {Gastroenterology},
volume = {162},
number = {6},
issn = {0016-5085},
address = {Philadelphia, Pa. [u.a.]},
publisher = {Saunders},
reportid = {DKFZ-2022-00121},
pages = {1705-1715},
year = {2022},
note = {2022 May;162(6):1705-1715},
abstract = {A genome-wide significant association between
anti-Helicobacter pylori (H. pylori) IgG titers and
Toll-like receptor (TLR1/6/10) locus on 4p14 was
demonstrated for individuals of European ancestry, but not
uniformly replicated. We re-investigate this association in
an updated genome-wide association study (GWAS)
meta-analysis for low gastric cancer incidence populations,
address potential causes of cohort heterogeneity and explore
functional implications of genetic variation at the
TLR1/6/10 locus.The dichotomous GWAS $(25\%$ individuals
exhibiting highest anti-H. pylori IgG titers versus
remaining $75\%)$ included a discovery and replication
sample of respectively n=15,685 and n=9,676, all of European
ancestry. Longitudinal analysis of serological data was
performed on H. pylori-eradicated subjects (n=132) and
patients under surveillance for premalignant gastric lesions
(n=107). TLR1/6/10 surface expression, TLR1 messenger RNA
(mRNA) and cytokine levels were measured in leukocyte
subsets of healthy subjects (n=26) genotyped for TLR1/6/10
variants.The association of the TLR1/6/10 locus with anti-H.
pylori IgG titers (rs12233670; β=-0.267 SE±0.034;
P=4.42x10-15) presented with high heterogeneity and failed
replication. Anti-H. pylori IgG titers declined within 2-4
years following eradication treatment (P=0.004), and
decreased over time in patients with premalignant gastric
lesions (P<0.001). Variation at the TLR1/6/10 locus affected
TLR1-mediated cytokine production and TLR1-surface
expression on monocytes (P=0.016) and neutrophils (P=0.030),
but not mRNA levels.The association between anti-H. pylori
IgG titers and TLR1/6/10 locus was not replicated across
cohorts, possibly due to dependency of anti-H. pylori IgG
titers on therapy, clearance and antibody decay. H.
pylori-mediated immune cell activation is partly mediated
via TLR1 signaling, which in turn is affected by genetic
variation.},
keywords = {Polymorphism (Other) / Single Nucleotide (Other) / bacteria
(Other) / immunity (Other) / serology (Other)},
cin = {C070},
ddc = {610},
cid = {I:(DE-He78)C070-20160331},
pnm = {313 - Krebsrisikofaktoren und Prävention (POF4-313)},
pid = {G:(DE-HGF)POF4-313},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:35031300},
doi = {10.1053/j.gastro.2022.01.011},
url = {https://inrepo02.dkfz.de/record/178524},
}